Team:UCLA/Notebook/Honeybee Silk/13 July 2015

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Colony PCR of 7/12 Transformation

• The transformation was successful for the 3:1 ligated products plates. I picked 3 colonies off the plate and suspended each in 100uL of ddH2O.
• Using APEX Taq Red Mastermix

Component	Volume (out of 25L)
APEX Taq Red Mastermix	12.5uL
10mM primer 10	2.5uL
10mM primer 11	1.25uL
Transformed Cells	1uL
Nuclease Free Water	9uL