Team:UCLA/Notebook/Protein Cages/12 July 2015

iGEM UCLA




Introduction: Starter culture will be done today.

Procedures:

10mL of LB was added to a culture tube. For a final concentration of 100ug/mL of carbenicillin, 10uL of 100mg/mL was added to the 10mL of LB. Using a sterile pipette tip, and pipette, a single colony was streaked and the entire tip was ejected into the culture tube. The tube was then incubated at 37C at 5:50PM. In addition, another 1L of LB was made and autoclaved for 30 minutes.