Team:UCLA/Notebook/Protein Cages/7 August 2015
Introduction: Today gel extraction and digestion of PCquad 3.0 will be done. If permitting, ligation will also be done.
Procedures: Gel extraction was performed as indicated by zymo procedures. The concentration was measured using the nanodrop. 78.78ng/uL. 260/280 = 3.23.
Since the yield was too low, another round of amplification will need to be done before digestion.
Conclusions: There weren’t any restriction enzymes available to use, so digestion will be done next week. PCR amplification will be done in a larger volume for PCquad 3.0 as well.