Team:UCLA/Notebook/Honeybee Silk/12 August 2015
Multiple Mini Expressions: Optimizing protein yield
- Today we are testing multiple growth conditions of our bl21 cells for total cell and protein yield. Parameters we are optimizing are iptg concentration, and growth temperature.
IPTG concentration/Temp | 37C | 30C |
---|---|---|
0.1 mM | ||
0.5 mM | ||
1.0 mM | ||
2.0 mM |