Difference between revisions of "Team:UCLA/Notebook/Recombinant Expression/29 May 2015"
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Revision as of 20:17, 29 May 2015
- Starter Culture Failure #3 : Back in Black
- Starter cultures grown on Wednesday were taken from the incubator at 1300 hours (~48 hours) and OD600 was taken on the samples.
- Tamura Plate 3 -- OD600: 1.273, Tamura Plate 4-- OD600: 1.246, Tamura Plate 5: OD600: 1.279.
- Starter cultures grown on Wednesday were taken from the incubator at 1300 hours (~48 hours) and OD600 was taken on the samples.
- Plasmid was mini prepped using the Promega PureYield Plasmid Miniprep System
- Plasmid ran on gel to verify size of plasmid, sequenced to verify proper insert, the will be used to transform into BL21(DE3) cells.