Difference between revisions of "Team:Aix-Marseille/Notebook"

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<p>Production of “01-1202” : Sonication of cells, centrifugation 10 min at 4500 rpm, ultracentrifugation 45 min at 4500 rpm, solubilizing membrane in Triton 1% during 2h and incubation overnight.</p>
 
<p>Production of “01-1202” : Sonication of cells, centrifugation 10 min at 4500 rpm, ultracentrifugation 45 min at 4500 rpm, solubilizing membrane in Triton 1% during 2h and incubation overnight.</p>
 
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<p>Plasmid purification of “45” “01-36” “381302” “38” “01-1202” “3812-02” “3813-02”</p>
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<p>Preparation of SDS Page 15% and a western blot with products of yesterday => results are not good so we have to resart the production</p>
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<p>Culture of “01-1202” and “01-3002” in anaerobic condition, cells were centrifuged 10 min at 4500 rpm and pellet were stored at -20°C</p>
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<p>A western blot was done → Production of Cytochrome, monomer in the membrane and dimer in the cytoplasm</p>
  
<p>Colony PCR of “47” “01-3002” “43” “44” => almost everything is positive!!! Great day!</p>
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<p>The next step is to purify “01-1202” and “01-3002” and start a culture on 01-3502 in anaerobic condition</p>
 
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<p>Preparation of SDS Page 15% and a western blot with products of yesterday => results are not good so we have to resart the production</p>
 
 
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Revision as of 16:21, 16 September 2015

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Chew figth project, for the iGEM competition. See you soon in Boston !