Team:UCLA/Notebook/Spider Silk Genetics/17 July 2015
iGEM UCLA
7/17/2015
Miniprep
- Made glycerol stocks of cells from yesterday.
- Miniprepped samples using Zymo Classic Kit.
|
|
Concentration (ng/uL)
|
A 260/280
|
| M2-3(T7)
|
620.9
|
1.86
|
|
|
569.58
|
1.86
|
|
|
453.28
|
1.84
|
| M2-6(T7)
|
519.13
|
1.84
|
|
|
434.09
|
1.81
|
|
|
353.01
|
1.83
|
| M2-9(1C3)
|
279.04
|
1.82
|
|
|
268.05
|
1.80
|
|
|
214.68
|
1.83
|
- M2 denotes MaSp type.
- The number denotes number of monomer repeats.
- Parentheses denotes the plasmid that the repeat is cloned in.
- Can be in pSB1C3 or BBa_K525998
Sequencing
- Sent samples for sequencing to genewiz.
End-Extension Primers for MaSp1 AB, BC, CA
- We ordered 6 primers today for MaSp1.
