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| − | <h2> Welcome to iGEM 2015! </h2> | + | <h2> Light-induced miRNA expression and heat-induced lysis in <i>E. coli</i> </h2> |
| − | <p>Your team has been approved and you are ready to start the iGEM season! </p> | + | |
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| − | <h4>Before you start: </h4> | + | <p>The honeysuckle plant (<i>Lonicera japonica</i>) has been used against influenza infections in traditional chinese medicine for hundreds of years, but only last year the active compound, the microRNA 2911, has been identified (Zhou, Li, Liu, Dong <i>et al.</i> Cell Research 2015). The mechanism remains to be verified, but it seems to interfere with genes from most influenza A virus strains, inhibiting their replication.</p> |
| − | <p> Please read the following pages:</p> | + | |
| − | <ul>
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| − | <li> <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
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| − | <li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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| − | </ul>
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| − | <div class="highlightBox">
| + | <p>While the traditional way of producing the compound is to grow the plant, dry the leaves, cook a bitter decotion from them and drink that decotion, we want to establish a biotechnological production in <i>E. coli</i>. The production shall furthermore be induced by light, for which we plan to use BioBricks from the Registry like the Cph8-EnvZ system.</p> |
| − | <h4> Styling your wiki </h4>
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| − | <p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p> | + | |
| − | <p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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| − | </div>
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| − | <h4> Editing your wiki </h4>
| + | <p>After our bacteria have produced enough of the miRNA, we want them to lyse themselves after a heat-induction. We plan on using the GroEL promoter in combination with a lysing cytotoxin, which remains to be chosen.</p> |
| − | <p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p> | + | |
| − | <p> <a href="https://2015.igem.org/wiki/index.php?title=Team:Hamburg&action=edit"> Click here to edit this page! </a></p>
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| − | <p>See tips on how to edit your wiki on the <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation</a> page.</p>
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| − | <h4>Templates </h4>
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| − | <p> This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the
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| − | <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</p>
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| − | <h4>Tips</h4>
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| − | <p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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| − | <ul>
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| − | <li>State your accomplishments! Tell people what you have achieved from the start. </li>
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| − | <li>Be clear about what you are doing and how you plan to do this.</li>
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| − | <li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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| − | <li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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| − | <li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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| − | <li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
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| − | <li>Have lots of fun! </li>
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| − | </ul>
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| − | <h4>Inspiration</h4>
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| − | <p> You can also view other team wikis for inspiration! Here are some examples:</p>
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| − | <ul>
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| − | <li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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| − | <li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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| − | <li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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| − | </ul>
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| − | <h4> Uploading pictures and files </h4>
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| − | <p> You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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| − | When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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| − | <a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
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| | + | <p>After the bacteria have lysed themselves, the miRNA could in principle be extracted from the solution and applied as a drug against influenza A infections.</p> |
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Add a banner to your wiki!
You can make the image 980px by 200px
Remember to call the file: "Team_Hamburg_banner.jpg"
Light-induced miRNA expression and heat-induced lysis in E. coli
The honeysuckle plant (Lonicera japonica) has been used against influenza infections in traditional chinese medicine for hundreds of years, but only last year the active compound, the microRNA 2911, has been identified (Zhou, Li, Liu, Dong et al. Cell Research 2015). The mechanism remains to be verified, but it seems to interfere with genes from most influenza A virus strains, inhibiting their replication.
While the traditional way of producing the compound is to grow the plant, dry the leaves, cook a bitter decotion from them and drink that decotion, we want to establish a biotechnological production in E. coli. The production shall furthermore be induced by light, for which we plan to use BioBricks from the Registry like the Cph8-EnvZ system.
After our bacteria have produced enough of the miRNA, we want them to lyse themselves after a heat-induction. We plan on using the GroEL promoter in combination with a lysing cytotoxin, which remains to be chosen.
After the bacteria have lysed themselves, the miRNA could in principle be extracted from the solution and applied as a drug against influenza A infections.