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- <div id="1-1-pcr"> ...25µl reactions were run according to the PCR protocol <a href="#">here</a>500 KB (35,542 words) - 16:53, 18 September 2015
- <div id="1-1-pcr"> ...25µl reactions were run according to the PCR protocol.530 KB (37,526 words) - 03:47, 19 September 2015
- <div id="1-1-pcr"> ...25µl reactions were run according to the PCR protocol <a href="#">here</a>375 KB (27,047 words) - 18:13, 2 September 2015
- need to use high efficiency promoters and lower efficiency RBS. Justin will color:black'>PCR</span></b></p>333 KB (47,812 words) - 07:49, 18 September 2015
- ...h technics, supervised by the instructors. We learned basics, as running a PCR, and where we can find everything needed to work in the lab. Training sessions for PCR56 KB (6,991 words) - 00:06, 19 September 2015
- <div id="1-1-pcr"> ...25µl reactions were run according to the PCR protocol <a href="#">here</a>166 KB (11,675 words) - 10:51, 20 August 2015
- Introduction: Yesterday, Nithin performed a PCR amplication of our PCquad optimized for iGEM 2.0 with the primers to add th PCR amplification of construct attempt #27 KB (1,116 words) - 23:29, 17 July 2015
- allows for quick growth and high yields. Therefore the proper preparation of LB will be crucial to too high – we want to prevent spills.</li>97 KB (16,302 words) - 04:31, 20 November 2015
- <p><strong>Caution:</strong> Gel box has high voltage! Be sure to turn off power pack and unplug leads before removing li Colony PCR Protocols for Transformed <i>E.coli</i>68 KB (11,180 words) - 07:32, 18 September 2015
- <li><a href="#colonypcr">Colony PCR</a></li> ...ropdown-toggle" data-toggle="dropdown" href="#">Polymerase Chain Reaction (PCR)<span class="caret"></span></a>52 KB (3,960 words) - 11:11, 17 August 2015
- <li>PCR for all the single and composite gene parts from 2014 using the protocol sh <li>Q5 high fidelity polymerase 0.25ul</li>36 KB (5,999 words) - 03:43, 19 September 2015
- * gradient PCR using J04450 in pSB1C3 (#ZWDH#) as the template (diluted 1:10) primers are: * run the agarose gel of yesterdays gradient PCR products (#ES4X#) (expected length: 2070 bp)21 KB (3,097 words) - 19:20, 18 September 2015
- <li><a href="#pcr">Polymerase Chain Reaction (PCR)</a> <li><a href="#colonypcr">Colony PCR</a></li>54 KB (5,322 words) - 23:45, 16 September 2015
- <br><a href="#P12">12.PCR Protocols</a> <br><a href="#P13">13.PCR MasterMix</a>30 KB (5,210 words) - 11:44, 18 September 2015
- <li>PCR (Thermo Scientific Phusion High Fidelity PCR Master-mix):<br> <b>Protocol for PCR reaction:</b><br>18 KB (2,876 words) - 00:23, 19 September 2015
- ...ge"><b><u>Goal:</u></b></font> To amplify the Gaussia Luciferase DNA using PCR ...<div class = "indentlist"><ul>Use 5µl in PCR</ul></div>72 KB (10,158 words) - 20:59, 20 November 2015
- <li><p>PCR amplified todE insert and todF insert using FroggaBio 2X Taq FroggaMix</p> <li>Ran PCR purification of TodE and TodF according to the PureLink PCR Purification protocol.</li>21 KB (3,547 words) - 04:32, 20 November 2015
- ...="window.scrollTo(0,document.getElementById('fourth2').offsetTop)">4.2 Taq PCR</li> ...="window.scrollTo(0,document.getElementById('fourth3').offsetTop)">4.3 Pfu PCR</li>107 KB (14,954 words) - 19:51, 18 September 2015
- * Nucleospin Gel and PCR clean-up # Heat in microwave for 1 min at high power.13 KB (2,103 words) - 17:03, 18 September 2015
- ...k() { }); return false;"><h1>PCR product purification using QIAquick® PCR Purification Kit (QIAGEN)</h1></a> ...; Add 5 volumes Buffer PB to 1 volume of the PCR reaction and mix.</p>117 KB (15,375 words) - 00:00, 19 September 2015
- <li><a href="#directedmutagenesispcr">Directed mutagenesis PCR for restriction site elimination using a plasmid template </a></li> <li><a href="#pcramplification1">PCR amplification (applied to lcp)</a></li>24 KB (2,967 words) - 23:52, 20 November 2015
- <li>1μl dye to 5μl DNA is sufficient for PCR products and restriction digests.</li> <h4>PCR Components</h4>26 KB (2,428 words) - 03:18, 19 September 2015
- ...e combined <i>mamW+RFP+laccase</i> and <i>RFP</i>+<i>laccase</i> by fusion PCR (Fig. 1B).<br> ...amW</i> and <i>RFP</i> and <i>laccase</i> were amplified by PCR using high fidelity DNA polymerase. <strong>(B)</strong>36 KB (3,710 words) - 02:48, 19 September 2015
- ...style="width:500px; margin-top:-25px;"><h3>PCR Protocol using Phusion High-Fidelity DNA Polymerase</h3></div> <p><strong>PCR </strong><br />8 KB (1,052 words) - 05:45, 22 August 2015
- <li>Pursue the protocol by the NucleoSpin® Gel and PCR Clean-up protocol</li> <h4>3. PCR amplification</h4>17 KB (2,170 words) - 23:29, 18 September 2015
- ...CGCTACTAGTA) Primers. After that, PCR products were purified with QIAquick PCR Purification Kit. <br> <h3>PCR mixture </h3>5 KB (804 words) - 04:39, 16 September 2015
- ...nealing and extension, simulating <i>in vivo</i> DNA replication progress. PCR has radically altered molecular biology; via this technique, a trace amount ...ously. All these methods need a thermal cycler; Nested PCR and Multiplexed PCR need more than two pairs of primers, which may lead to the generation of pr39 KB (4,417 words) - 14:54, 17 November 2015
- <p class="text"><a href="#2206c">PCR Set-up</a></p> ...text"><a href="#2206d">Setting up Agarose Gel for Electrophoresis of 22/06 PCR Products</a></p>14 KB (1,920 words) - 15:44, 6 July 2015
- ...of PoxB, Pta, sdhA and some other enzymes could be knocked out to ensure a high succinic acid yield <sup>[15]</sup>. <!-- Error-Prone PCR -->43 KB (6,183 words) - 20:20, 18 September 2015
- <li>PCR'd off template</li> <p>PCR Reaction:</p>12 KB (1,619 words) - 23:38, 20 May 2015
- ...ks LuxI-His, RBS-LuxI-His and CheZ-GFP, RBS-CheZ-GFP by high fidelity tail-PCR, digestion and ligation in pSB1C3<br/> ...>, testing colonies by PCR and preparing for sequencing. The high fidelity PCR and cloning of other potential BioBricks was repeated. <br/>58 KB (8,236 words) - 10:16, 20 October 2015
- <h1 class="sectionedit1"><a name="pcr" id="pcr">PCR</a></h1> <!-- EDIT1 SECTION "PCR" [1-19] -->5 KB (690 words) - 07:05, 20 November 2015
- <h6> 12. Add 50 uL PCR water to the center of the QIAprep spin column to elute DNA, let stand for <h2>PCR using Q5 High-Fidelity DNA Polymerase</h2>25 KB (3,384 words) - 14:18, 17 September 2015
- <h6> 12. Add 50 uL PCR water to the center of the QIAprep spin column to elute DNA, let stand for <h2>PCR using Q5 High-Fidelity DNA Polymerase</h2>31 KB (4,008 words) - 12:01, 14 September 2015
- = PCR Amplification and Gel Extraction of InterLab Devices = == PCR Reaction & Thermocycler Setup ==14 KB (1,900 words) - 01:48, 16 August 2015
- <div class="textBox" id="PCR"> <h3>PCR protocol</h3>37 KB (5,389 words) - 10:32, 19 November 2015
- ='''Colony PCR of 5/4 Transformation'''= ====Colony PCR Reaction====11 KB (1,333 words) - 06:25, 10 July 2015
- <h4> 1) PCR Amplification using TaKaRa Ex Taq DNA polymerase</h4> <li> Set up the following cycles in a PCR machine42 KB (6,658 words) - 21:57, 19 November 2015
- <h3 class="sectionedit4">PCR</h3> ...CR approaches were used. In general, PCRs were performed with Phusion High Fidelity DNA Polymerase (NEB). The components of the reaction mixture were used in d19 KB (3,058 words) - 16:56, 28 August 2015
- ...="button" class="btn btn-info" data-toggle="collapse" data-target="#demo9">PCR protocol with EconoTaq PLUS 2X</button> ...fo" data-toggle="collapse" data-target="#demo10">PCR protocol with Q5 High Fidelity DNA Polymerase</button>31 KB (3,932 words) - 00:07, 19 September 2015
- ...ectively). Then dsDNAs was amplified with four sets of specific primers by PCR. It went through smoothly. So far, we got the target fragments successfully <div style="text-align:left">We picked the colonies to do PCR and sent the positive sequencing(M13).</div>18 KB (2,547 words) - 21:19, 18 September 2015
- ...the RBS each with only one different nucleotide are designed to carry out PCR. The kit we used allows the whole plasmid to be replicated and the product ...m <i>Psedomonas aeruginosa</i> work well in the chassis of MR-1, giving a high level of electrical output.20 KB (2,725 words) - 03:32, 19 September 2015
- <h2 style="float:left;margin-left:10px;clear:right;" >Error-prone PCR</h2> ...ng the fidelity of the DNA polymerase. This process is similar to standard PCR cloning of a target sequence except that, for constructing a diverse mutage7 KB (948 words) - 20:51, 18 September 2015
- <tr><td>PCR cycler</td><td>Gene Amp PCR System 9700</td><td>Applied Biosystems</td><td>Waltham</td><td>USA</td></tr <tr><td>Phusion High-GC Buffer</td><td>NEB (New England Biolabs)</td><td>Ipswitch</td><td>USA</t14 KB (2,470 words) - 07:00, 20 November 2015
- ='''Gel Visualization of 5/26 PCR'''= The PCR products from 5/26 were run on a 1% agarose gel against a 1kB ladder. I use10 KB (1,234 words) - 22:32, 16 July 2015
- ...tes, we obtained the double knock-outs. These knock-outs were confirmed by PCR. For more information, please check our result page. <br/> ...nzymes are needed: a mesophylic nuclease, a thermophylic ligase and a high fidelity polymerase. Therefore, the NEBuilder<sup>Ⓡ</sup> HiFi DNA Assembly Ma45 KB (6,730 words) - 09:34, 20 October 2015
- ...a lower percentage, such as 0.7% gel, is best for gel extractions, and as high as 2% agarose can be used when resolving large bands of DNA.</li> <h2>I. PCR for Amplification.</h2>10 KB (1,713 words) - 03:10, 19 September 2015
- <summary>4/26 PCR off honey bee gene block</summary> <p>PCR Reaction 1 (q5 polymerase kit): primers honeybee p#7 and honeybee p#8 with4 KB (359 words) - 16:33, 5 June 2015
- <summary> 4/28 PCR to prepare 2 honeybee constructs for cloning</summary> We are repeating the same PCR from 4/26, but only at one temperature instead of a gradient, and we are sc3 KB (321 words) - 16:35, 5 June 2015
- ='''Colony PCR of 7/15 Transformation'''= ===Colony PCR Reaction===10 KB (1,301 words) - 01:58, 17 July 2015
- <strong>PCR of inserts for cloning - Phusion DNA polymerase</strong> <p>To amplify an insert for cloning, using high-fidelity phusion polymerase.</p>4 KB (433 words) - 21:43, 31 August 2015
- ='''Picking colonies and Colony PCR of 7/17 Transformation'''= ===Colony PCR Reaction===11 KB (1,433 words) - 20:16, 20 July 2015
- ='''PCR of Honeybee G-block and Purification'''= ...ion and purification was too low (20.12ng/uL), today I will be redoing the PCR, digestion, and purification to obtain a higher yield.11 KB (1,323 words) - 20:14, 15 July 2015
- ='''PCR of Lac promoter/Silk/Spycatcher'''= !Q5 High Fidelity DNA Polymerase11 KB (1,451 words) - 21:47, 24 August 2015
- <td><b>PCR protocol (Phusion)</b><br /> <li>25µL of <i>Life Technologies</i> Phusion High-Fidelity PCR Master Mix with HF Buffer</li>1 KB (176 words) - 16:05, 1 September 2015
- 0.5 uL of Phusion High-Fidelity DNA Polymerase (Thermofisher) - added last<br> The PCR products of both the insert and the linearized plasmid were then combined i25 KB (3,709 words) - 05:04, 21 December 2015
- ...d Suffix-R Primers. After that, pcr products were cleaned up with QIAquick PCR Purification Kit. PCR- reaction4 KB (520 words) - 01:28, 9 September 2015
- ...as subjected to reverse transcription PCR (RT-PCR) using Transcriptor High Fidelity cDNA Synthesis Kit (Roche,Penzberg, Germany) to generate cDNA.</br> The PCR segments were digested with specific enzymes and ligated with linearized pc9 KB (1,271 words) - 02:41, 19 September 2015
- <li>PCR'd off template</li> <p>PCR Reaction:</p>9 KB (1,187 words) - 07:18, 14 July 2015
- ='''PCR of Honeybee G-Block'''= *Diagnostic PCR, will be running a temperature gradient14 KB (1,912 words) - 20:39, 21 July 2015
- <h1>PCR with Phusion-HF DNA Polymerase (NEB):</h1> Using the guidelines of the provided Phusion® High-Fidelity DNA Polymerase (M0530)3 KB (345 words) - 12:09, 18 September 2015
- <li>Product: Q5® High-Fidelity 2X Master Mix </li> <a href="https://www.neb.com/products/m0492-q5-high-fidelity-2x-master-mix"><p>for further information click here</p></a>27 KB (4,268 words) - 23:53, 18 September 2015
- ...yle="color:#660066">Sigma Aldrich</span></b> is a leading Life Science and High Technology company focused on enhancing human health and safety, manufactur ...isms, amplify them and then produce the assemblies by overlap extension by PCR. Thanks to gBlocks we could save all this work in laboratory that would req41 KB (5,516 words) - 17:07, 14 November 2015
- ...yle="color:#660066">Sigma Aldrich</span></b> is a leading Life Science and High Technology company focused on enhancing human health and safety, manufactur ...isms, amplify them and then produce the assemblies by overlap extension by PCR. Thanks to gBlocks we could save all this work in laboratory that would req41 KB (5,516 words) - 17:58, 14 November 2015
- ...align:left">PCR amplification of BBa_K936011 and BBa_K936023 using Q5 High fidelity Master Mix.</li>9 KB (1,123 words) - 02:18, 19 September 2015
- ...es compatible with the RDP standard, they were amplified via high fidelity PCR with the primers that we designed previously. The templates were the coding5 KB (829 words) - 20:46, 18 September 2015