Team:Paris Bettencourt/Protocols/Analytical digestion

Ferment It Yourself

iGEM Paris-Bettencourt 2O15

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Notebook

Vitamin A	Vitamin B2	Vitamin B12
Phytase	Riboswitch	Differentiation on E. coli
Differentiation on S. cerevisiae	Manufacturing	Idli and Micro-organisms

Analytical digestion protocol Prepare the following mix: 2µL 10X Digestion buffer 0.5µL Eco31I 0.5µL BbsI 2µL of DNA (200ng) 15µL water incube 1h at 37°C