Team:Paris Bettencourt/Protocols/Digestion

Ferment It Yourself

iGEM Paris-Bettencourt 2O15

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Notebook

Vitamin A	Vitamin B2	Vitamin B12
Phytase	Riboswitch	Differentiation on E. coli
Differentiation on S. cerevisiae	Manufacturing	Idli and Micro-organisms

Digestion Protocol Prepare the following mix: 4μL of Enzyme 1 Fast Digest 4μL of Enzyme 2 Fast Digest 4μL of FastAP 12μL of Fast Digest buffer 10X 1 to 3 μg of DNA up to 120μL of water mix by pipetting up and down incube 10 to 20 minutes at 37°C incube 10 minutes at 68°C to inactivate the enzymes