Team:Paris Bettencourt/Protocols/Electroporation
Ferment It Yourself
iGEM Paris-Bettencourt 2O15
- Background
- Design
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Notebook
Vitamin A Vitamin B2 Vitamin B12 Phytase Riboswitch Differentiation on E. coli Differentiation on S. cerevisiae Manufacturing Idli and Micro-organisms Electroporation Protocol
- Thaw electrocompetent cells on ice
- Add 2µL of ligation product or 0.5µL of native plasmid to the cells
- Transfer the cells in an 0.2mm electroporation cuvette
- put the cuvette in the electroporation device and pulse the cells at 2.5kV, 200 Ohms and 25µF
- add 200µL of LB right after pulsing
- recover 2 hours at 37°C
- plate 200µL and 50µL of the cells on LB + erythromycin (200µg/mL), incubate overnight at 37°C