Difference between revisions of "Team:Paris Bettencourt/Notebook/Phytase"
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Result of plates:<br> | Result of plates:<br> | ||
− | + | <div class="column-left"><img src="https://static.igem.org/mediawiki/2015/b/b8/Paris-bettencourt-g%C3%A9lose65.png" width="300px"></div> | |
− | <div class="column-left">There is a culture in plates.<br> | + | <div class="column-right">There is a culture in plates.<br> |
The negative control is not good. The no change yeast grow in the YPD medium with the antibiotic. We will repeat this control on an agar plate and not in a liquid medium.<br> | The negative control is not good. The no change yeast grow in the YPD medium with the antibiotic. We will repeat this control on an agar plate and not in a liquid medium.<br> | ||
− | We analyze anyway down results, the results of the new control will allow us to validate the result of our experiment or search which are our error and try again.<br> | + | We analyze anyway down results, the results of the new control will allow us to validate the result of our experiment or search which are our error and try again.</div><br> |
− | We see more colonies on the plates with yeast transforming PHO85 and FRT+PHO85.<br> | + | |
+ | <div class="column-left">We see more colonies on the plates with yeast transforming PHO85 and FRT+PHO85.<br> | ||
We look only few colonies in the plates with yeast transforming PHO80.<br></div> | We look only few colonies in the plates with yeast transforming PHO80.<br></div> | ||
− | <div class="column-right"><img src="https://static.igem.org/mediawiki/2015/b/b8/Paris-bettencourt-g%C3%A9lose65.png" width=" | + | <div class="column-right"><img src="https://static.igem.org/mediawiki/2015/b/b8/Paris-bettencourt-g%C3%A9lose65.png" width="300px"></div> |
− | <br> | + | <br><br> |
Verification of the results thanks to the colony PCR, to determinate if the resistance is integrated.<br> | Verification of the results thanks to the colony PCR, to determinate if the resistance is integrated.<br> | ||
Create the primer:<br> | Create the primer:<br> |
Revision as of 08:26, 19 August 2015