Difference between revisions of "Team:Paris Bettencourt/Notebook/Manufacturing"
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<div id="notebookContent"> | <div id="notebookContent"> | ||
<a name="july" class="anchor"><h1></h1></a> | <a name="july" class="anchor"><h1></h1></a> | ||
− | <h1 class="date one">July 27th</h1> | + | <h1 class="date one">July 27th</h1><br> |
Beginning of the manufacturing project! Let's do it!<br> | Beginning of the manufacturing project! Let's do it!<br> | ||
− | + | ||
− | + | ||
− | + | ||
<h2>Goal</h2> | <h2>Goal</h2> | ||
+ | Choice of the strain of yeast: Sc. M Cherry, wich is resistant to geneticin at a concentration of 200µL/mL, and produce RFP<br> | ||
We will have to calculate a survival rate so we have to be able to know how many cells we have in the first place for each experiment. | We will have to calculate a survival rate so we have to be able to know how many cells we have in the first place for each experiment. | ||
<h2>Procedure</h2> | <h2>Procedure</h2> | ||
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<li>Make dilutions of the yeasts/water solution to plate them</li> | <li>Make dilutions of the yeasts/water solution to plate them</li> | ||
<li>Deduce the concentration of Sc. M Cherry corresponding to 1 OD</li> | <li>Deduce the concentration of Sc. M Cherry corresponding to 1 OD</li> | ||
− | </ul> | + | </ul><br> |
− | First try today and plating of the different dilutions on YPD agar+ GEN (200) | + | First try today and plating of the different dilutions on YPD agar+ GEN (200) |
− | + | ||
− | + | ||
<br><br> | <br><br> | ||
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<h2>Results</h2> | <h2>Results</h2> | ||
The aluminium is not a good idea, the yeasts stick to it and it gets destroyed when we scratch.<br> | The aluminium is not a good idea, the yeasts stick to it and it gets destroyed when we scratch.<br> | ||
− | We have to find another drying paper. | + | We have to find another drying paper. |
<br><br> | <br><br> | ||
<h1 class="date one">July 29th</h1> | <h1 class="date one">July 29th</h1> | ||
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The 10mL solution of yeasts/water had an OD of 2,557 and 100µL of the 10^-4 dilution grew 359 colonies, so:<br> | The 10mL solution of yeasts/water had an OD of 2,557 and 100µL of the 10^-4 dilution grew 359 colonies, so:<br> | ||
<b>1 OD = 1,4x10^7 cells/mL</b><br> | <b>1 OD = 1,4x10^7 cells/mL</b><br> | ||
− | We launched a similar experience for more repetitions. | + | We launched a similar experience for more repetitions. |
<br><br> | <br><br> | ||
<h1 class="date one">July 30th</h1> | <h1 class="date one">July 30th</h1> | ||
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Yeasts grew in the plate. We can check that they produce RFP, it's Sc. M Cherry who survived.<br> | Yeasts grew in the plate. We can check that they produce RFP, it's Sc. M Cherry who survived.<br> | ||
Let's repeat the experience but drying the yeasts on a plastic dish.<br> | Let's repeat the experience but drying the yeasts on a plastic dish.<br> | ||
− | This time, we took the OD and plated a known quantity of the obtained powder to be able to calculate the survival rate. | + | This time, we took the OD and plated a known quantity of the obtained powder to be able to calculate the survival rate. |
<br><br> | <br><br> | ||
<h1 class="date one">July 31th</h1> | <h1 class="date one">July 31th</h1> | ||
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<br> | <br> | ||
<b>Second idea: Cooking a powder</b><br> | <b>Second idea: Cooking a powder</b><br> | ||
− | < | + | <h2>Procedure (found here: http://www.instructables.com/id/Stop-Paying-for-Yeast-Make-Your-Own/)</h2> |
<ul> | <ul> | ||
<li>Cook some potatoes</li> | <li>Cook some potatoes</li> | ||
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</ul> | </ul> | ||
We decided to try with wheat and rice flour to see if there is a difference. Since the product is designed for rice fermentation, using rice flour would be better!<br> | We decided to try with wheat and rice flour to see if there is a difference. Since the product is designed for rice fermentation, using rice flour would be better!<br> | ||
− | We also tried with sugar and ginger, then with only sugar and then without any sugar nor ginger.<br> | + | We also tried with sugar and ginger, then with only sugar and then without any sugar nor ginger. |
− | <br> | + | <br><br> |
− | < | + | <h1 class="date two">August 3rd</h1> |
<i>Results of the second drying:</i><br> | <i>Results of the second drying:</i><br> | ||
The survival rate is about <b>70%</b>.<br> | The survival rate is about <b>70%</b>.<br> |
Revision as of 12:27, 4 September 2015