Difference between revisions of "Team:Paris Bettencourt/Notebook/Manufacturing"
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First try today and plating of the different dilutions on YPD agar+ GEN (200) | First try today and plating of the different dilutions on YPD agar+ GEN (200) | ||
− | <br> | + | <br><br> |
<h1 class="date one">July 28th</h1> | <h1 class="date one">July 28th</h1> | ||
<h2>Goal</h2> | <h2>Goal</h2> | ||
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<br><br> | <br><br> | ||
<h1 class="date one">July 29th</h1> | <h1 class="date one">July 29th</h1> | ||
− | <H2>Result of the first OD test:</h2 | + | <H2>Result of the first OD test:</h2> |
The 10mL solution of yeasts/water had an OD of 2,557 and 100µL of the 10^-4 dilution grew 359 colonies, so:<br> | The 10mL solution of yeasts/water had an OD of 2,557 and 100µL of the 10^-4 dilution grew 359 colonies, so:<br> | ||
<b>1 OD = 1,4x10^7 cells/mL</b><br> | <b>1 OD = 1,4x10^7 cells/mL</b><br> | ||
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<br><br> | <br><br> | ||
<h1 class="date one">July 30th</h1> | <h1 class="date one">July 30th</h1> | ||
− | <h2>Results of the drying test:</h2 | + | <h2>Results of the drying test:</h2> |
Yeasts grew in the plate. We can check that they produce RFP, it's Sc. M Cherry who survived.<br> | Yeasts grew in the plate. We can check that they produce RFP, it's Sc. M Cherry who survived.<br> | ||
Let's repeat the experience but drying the yeasts on a plastic dish.<br> | Let's repeat the experience but drying the yeasts on a plastic dish.<br> | ||
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<br><br> | <br><br> | ||
<h1 class="date one">July 31th</h1> | <h1 class="date one">July 31th</h1> | ||
− | <h2>Result of the second OD test:</h2 | + | <h2>Result of the second OD test:</h2> |
??????????????????????????????????? | ??????????????????????????????????? | ||
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<br><br> | <br><br> | ||
<h1 class="date two">August 3rd</h1> | <h1 class="date two">August 3rd</h1> | ||
− | <h2>Results of the second drying test:</h2 | + | <h2>Results of the second drying test:</h2> |
The survival rate is about <b>70%</b>.<br> | The survival rate is about <b>70%</b>.<br> | ||
<h2>Procedure</h2> | <h2>Procedure</h2> | ||
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<br><br> | <br><br> | ||
<h1 class="date two">August 6th</h1> | <h1 class="date two">August 6th</h1> | ||
− | <h2>Results of the cube test:</h2 | + | <h2>Results of the cube test:</h2> |
The survival rate is about 6%. | The survival rate is about 6%. | ||
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<br><br> | <br><br> | ||
<h1 class="date two">August 10th</h1> | <h1 class="date two">August 10th</h1> | ||
− | <h2>Results of the OD test for G1513:</h2 | + | <h2>Results of the OD test for G1513:</h2> |
????????????????????????????<br> | ????????????????????????????<br> | ||
<br> | <br> |
Revision as of 12:53, 4 September 2015