Difference between revisions of "Team:Paris Bettencourt/Notebook/Manufacturing"
CeliaFoulon (Talk | contribs) |
CeliaFoulon (Talk | contribs) |
||
Line 175: | Line 175: | ||
<h1 class="date two">August 12th</h1> | <h1 class="date two">August 12th</h1> | ||
<h2>Results of the cube tests</h2> | <h2>Results of the cube tests</h2> | ||
− | The yeasts survived well: 42%. But strangely, the number of cells which are supposed to be Lactococcus, in the plates of M17 glucose with erythromycin, is the same as in the plates with YPD and geneticin, selecting the yeasts.<br> | + | The yeasts survived well: 42%. <br> |
+ | But strangely, the number of cells which are supposed to be Lactococcus, in the plates of M17 glucose with erythromycin, is the same as in the plates with YPD and geneticin, selecting the yeasts.<br> | ||
Therefore, we checked the production of RFP, since Sc. M Cherry produces RFP but not G1513.<br> | Therefore, we checked the production of RFP, since Sc. M Cherry produces RFP but not G1513.<br> | ||
Unfortunately, we came to the conclusion that the cells in the M17 plates were yeasts. Actually, Sc. M Cherry resist to erythromycin and is therefore growing in the plate.<br> | Unfortunately, we came to the conclusion that the cells in the M17 plates were yeasts. Actually, Sc. M Cherry resist to erythromycin and is therefore growing in the plate.<br> | ||
Line 188: | Line 189: | ||
We had the idea of adding sugar, to see if it changes something.<br> | We had the idea of adding sugar, to see if it changes something.<br> | ||
The potato juice/rice flour media strongly smells yeasts. We therefore admit the yeast grew in this media but decided that the aspect wasn't very attractive and the smell so so bad that indian population would be disgusted and won't use it. | The potato juice/rice flour media strongly smells yeasts. We therefore admit the yeast grew in this media but decided that the aspect wasn't very attractive and the smell so so bad that indian population would be disgusted and won't use it. | ||
− | |||
− | |||
− | |||
<br><br> | <br><br> | ||
<h1 class="date two">August 17th</h1> | <h1 class="date two">August 17th</h1> | ||
+ | <h2>Procedure/h2> | ||
+ | Making new cubes with Sc. M Cherry alone, G1513 alone, and a mix of both strains. <br> | ||
+ | Measuring the OD and plating to count the cells | ||
+ | |||
+ | <h2>Results of the media test</h2> | ||
+ | With sugar, we can see there are cells growing in the potato juice.<br> | ||
+ | It's a very small growth compared to the one in YPD but it's still a growth.<br> | ||
+ | We will work more precisely on this media, trying to deduce the influence of the concentration in potato and in sugar. | ||
+ | |||
<br><br> | <br><br> | ||
<h1 class="date two">August 18th</h1> | <h1 class="date two">August 18th</h1> | ||
+ | <h2>Results of the OD test</h2> | ||
+ | For Sc. M Cherry:<br> | ||
+ | <b>1 OD = 3,8x10^6 cells/mL</b><br> | ||
+ | For G1513:<br> | ||
+ | <b>1 OD = 1,1x10^8 cells/mL</b><br> | ||
<br><br> | <br><br> | ||
<h1 class="date two">August 19th</h1> | <h1 class="date two">August 19th</h1> | ||
+ | <h2>Results of the cube test</h2> | ||
+ | <ul> | ||
+ | <li>Sc. M Cherry alone: 39%</li> | ||
+ | <li>Sc. M Cherry in the mix: 26%</li> | ||
+ | <li>G1513 alone: 1,8%</li> | ||
+ | </ul> | ||
+ | We kept samples of the mix in the fridge to be able to study it later, when we will have the cycloheximide. | ||
<br><br> | <br><br> | ||
<h1 class="date two">August 20th</h1> | <h1 class="date two">August 20th</h1> | ||
+ | <h2>Procedure</h2> | ||
+ | Making more cubes with Sc. M Cherry alone, G1513 alone, and a mix of both. | ||
<br><br> | <br><br> |
Revision as of 16:36, 8 September 2015