Difference between revisions of "Team:Paris Bettencourt/Notebook/Manufacturing"
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<h1 class="date one">July 29th</h1> | <h1 class="date one">July 29th</h1> | ||
<H2>Result of the first OD test:</h2> | <H2>Result of the first OD test:</h2> | ||
− | The | + | The 10ml solution of yeasts/water had an OD of 2,557 and 100µL of the 10^-4 dilution grew 359 colonies, so:<br> |
− | <b>1 OD = 1,4x10^7 cells/ | + | <b>1 OD = 1,4x10^7 cells/ml</b><br> |
We launched a similar experience for more repetitions. | We launched a similar experience for more repetitions. | ||
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<h1 class="date one">July 31th</h1> | <h1 class="date one">July 31th</h1> | ||
<h2>Result of the second OD test:</h2> | <h2>Result of the second OD test:</h2> | ||
− | <b>1 OD = 7,8x10^6 cells/ | + | <b>1 OD = 7,8x10^6 cells/ml</b> |
<br><br> | <br><br> | ||
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<h1 class="date two">August 10th</h1> | <h1 class="date two">August 10th</h1> | ||
<h2>Results of the OD test for G1513:</h2> | <h2>Results of the OD test for G1513:</h2> | ||
− | <b>1 OD = 6,0x10^4 cells/ | + | <b>1 OD = 6,0x10^4 cells/ml</b><br> |
<h2>Procedure</h2> | <h2>Procedure</h2> | ||
Making new cubes containing Sc. M Cherry and G1513 to calculate the survival rate. | Making new cubes containing Sc. M Cherry and G1513 to calculate the survival rate. | ||
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<h2>Results of the cube tests</h2> | <h2>Results of the cube tests</h2> | ||
The yeasts survived well: 42%. <br> | The yeasts survived well: 42%. <br> | ||
− | Seven days later, we plated again cubes from this test, and obtained a survival rate of 21%. | + | Seven days later, we plated again cubes from this test, and obtained a survival rate of 21%.<br> |
− | But strangely, the number of cells | + | But strangely, the number of cells in the plates of M17 glucose with erythromycin, which are supposed to be G1513, is the same as in the plates with YPD and geneticin, selecting the yeasts.<br> |
Therefore, we checked the production of RFP, since Sc. M Cherry produces RFP but not G1513.<br> | Therefore, we checked the production of RFP, since Sc. M Cherry produces RFP but not G1513.<br> | ||
Unfortunately, we came to the conclusion that the cells in the M17 plates were yeasts. Actually, Sc. M Cherry resist to erythromycin and is therefore growing in the plate.<br> | Unfortunately, we came to the conclusion that the cells in the M17 plates were yeasts. Actually, Sc. M Cherry resist to erythromycin and is therefore growing in the plate.<br> | ||
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<h2>Results of the OD test</h2> | <h2>Results of the OD test</h2> | ||
For Sc. M Cherry:<br> | For Sc. M Cherry:<br> | ||
− | <b>1 OD = 3,8x10^6 cells/ | + | <b>1 OD = 3,8x10^6 cells/ml</b><br> |
For G1513:<br> | For G1513:<br> | ||
− | <b>1 OD = 1,1x10^8 cells/ | + | <b>1 OD = 1,1x10^8 cells/ml</b><br> |
<br><br> | <br><br> | ||
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<h2>Results of the OD test</h2> | <h2>Results of the OD test</h2> | ||
For Sc. M Cherry:<br> | For Sc. M Cherry:<br> | ||
− | <b>1 OD = 3,1x10^6 cells/ | + | <b>1 OD = 3,1x10^6 cells/ml</b><br> |
For G1513:<br> | For G1513:<br> | ||
Nothing... It seems that the colony used was dead so the cubes will not contained any G1513.<br> | Nothing... It seems that the colony used was dead so the cubes will not contained any G1513.<br> | ||
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<br><br> | <br><br> | ||
<h1 class="date two">August 25th</h1> | <h1 class="date two">August 25th</h1> | ||
− | + | <h2>Results of the OD test</h2> | |
+ | For Sc. M Cherry:<br> | ||
+ | <b>1 OD = 2,6x10^6 cells/mL</b><br> | ||
+ | For G1513:<br> | ||
+ | Nothing... We used cells from the previous <br> | ||
<br><br> | <br><br> |
Revision as of 12:17, 9 September 2015