Difference between revisions of "Team:Paris Bettencourt/Notebook/Manufacturing"
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<div class="column-left"><p> | <div class="column-left"><p> | ||
<br> | <br> | ||
− | The result is a small solid cube, very | + | The result is a small solid cube, very easy to give away.<br> |
Several cubes can be packed together.<br> | Several cubes can be packed together.<br> | ||
We tought it could be a good media of distribution.<br> | We tought it could be a good media of distribution.<br> | ||
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<h1 class="date two">August 6th</h1> | <h1 class="date two">August 6th</h1> | ||
<h2>Results of the cube test:</h2> | <h2>Results of the cube test:</h2> | ||
− | The survival rate is about 7%. | + | The survival rate is about 7%.<br> |
+ | After staying 5hours in the water, the survival rate reached 737%. | ||
<br><br> | <br><br> | ||
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<b>1 OD = 2,6x10^6 cells/mL</b><br> | <b>1 OD = 2,6x10^6 cells/mL</b><br> | ||
For G1513:<br> | For G1513:<br> | ||
− | Nothing... We used cells from the previous <br> | + | Nothing... We used cells from the previous culture, wich was dead, so it seems normal.<br> |
<br><br> | <br><br> | ||
<h1 class="date two">August 26th</h1> | <h1 class="date two">August 26th</h1> | ||
+ | <h2>Results of the cube test</h2> | ||
+ | <ul> | ||
+ | <li>Sc. M Cherry alone: 122% et 162%</li> | ||
+ | <li>Sc. M Cherry in the mix (but dead G1513): 178% et 217</li> | ||
+ | <li>G1513 alone: Nothing</li> | ||
+ | </ul> | ||
<br><br> | <br><br> | ||
<h1 class="date two">August 27th</h1> | <h1 class="date two">August 27th</h1> | ||
+ | <h2>Goal</h2> | ||
+ | We want to find a media that could be use in India to grow the yeasts and the Lactococcus lactis for cheap.<br> | ||
+ | <h2>Procedure</h2> | ||
+ | <ul> | ||
+ | <li>Make 3 different potato juice solutions: low, medium and high concentration in potatoes</li> | ||
+ | <li>For each one of these solutions, make samples with different concentration of sugar</li> | ||
+ | <li>Inoculate the strains separetely in these media, with the appropriate antibiotics</li> | ||
+ | <li>Put 150μL of the solutions in a Tecan plate well and add 50μL of oil</li> | ||
+ | <li>Put in the Tecan at 30°C, measuring the OD every 15minutes, for 2 days</li> | ||
+ | </ul> | ||
<br><br> | <br><br> | ||
<h1 class="date two">August 28th</h1> | <h1 class="date two">August 28th</h1> | ||
+ | <h2>Goal</h2> | ||
+ | We want to see how the strains in the cubes react when put in the idli.<br> | ||
+ | <h2>Procedure</h2> | ||
+ | ??????????????? | ||
<br><br> | <br><br> | ||
<h1 class="date two">August 31th</h1> | <h1 class="date two">August 31th</h1> | ||
+ | <h2>Results of the media test</h2> | ||
+ | It's a fail, the OD was too high so we can't really see the growth.<br> | ||
+ | We did it again, diluting the media and strain 200times in osmosed water.<br> | ||
+ | <h2>Results of the idli test</h2> | ||
+ | ???????????????????????????? | ||
<br><br> | <br><br> | ||
− | <h1 class="date three">September | + | <h1 class="date three">September 2nd</h1> |
+ | <h2>Results of the second media test</h2> | ||
+ | It's a fail, there was almost only water in the well so nothing grew<br> | ||
+ | We will do it again, asking for a real protocol to some experts.<br> | ||
<br><br> | <br><br> | ||
− | <h1 class="date three">September | + | <h1 class="date three">September 4th</h1> |
+ | <h2>Procedure</h2> | ||
+ | Trying the new method for the Tecan test.<br> | ||
+ | This time, we did an overnight culture for each media and each strain, that we diluted 200times (till we get an OD smaller than 0,01)<br> | ||
<br><br> | <br><br> | ||
− | <h1 class="date three">September | + | <h1 class="date three">September 7th</h1> |
+ | <h2>Results of the Tecan test</h2> | ||
+ | Third fail, because one parameter was changed and we didn't noticed: the Tecan measured the OD for only few hours, not enough to see the growth... | ||
<br><br> | <br><br> | ||
− | <h1 class="date three">September | + | <h1 class="date three">September 8th</h1> |
+ | <h2>Procedure</h2> | ||
+ | We made fresh new culture of Sc. M Cherry and G1513, that we used today to make lots of new cubes and OD test. | ||
+ | We planned on making many cubes to plate one each day and follow the survival rate during the week. | ||
+ | |||
+ | <br><br> | ||
+ | <h1 class="date three">September 9th</h1> | ||
+ | |||
+ | |||
+ | <br><br> | ||
+ | <h1 class="date three">September 10th</h1> | ||
</html> | </html> |
Revision as of 15:22, 9 September 2015