Difference between revisions of "Team:Paris Bettencourt/Project/Manufacturing"
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<img src="https://static.igem.org/mediawiki/2015/1/17/PBVitacube.png" width="666" height="470"><br> | <img src="https://static.igem.org/mediawiki/2015/1/17/PBVitacube.png" width="666" height="470"><br> | ||
</div> | </div> | ||
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<div class="column-left" align="justify"> | <div class="column-left" align="justify"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/f/fd/PBmanufacturingcube.jpg" height="321" width="512"> | ||
+ | </div> | ||
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Now that we have found a convenient distribution mean, we need to be sure that it keeps our strains alive and to know how long it can be stored.<br> | Now that we have found a convenient distribution mean, we need to be sure that it keeps our strains alive and to know how long it can be stored.<br> | ||
− | We conducted several survival test on the VitaCubes, using <i>S.cerevisiae</i> mCherry and <i>L.</i> lactis. To make these | + | We conducted several survival test on the VitaCubes, using <i>S.cerevisiae</i> mCherry and <i>L.</i> lactis. To make these tests, we were plating the liquid solution containing the strains before adding it to the flour in order to know how many cells we put in the VitaCube. After a define period of drying, we immerse the VitaCube in 10ml of osmosed water and plated the solution obtained to count the numbers of cells that survived in the cube.<br> At first, we obtained very different results, even if the cubes were all plated after one day of drying. We realised the time of immersion of the cube in the water had an influence over the survival rate: the longer it was, the bigger was the survival rate. This is due to the revival of cells once back in water.<br> |
Therefore, we designed a one week experiment: we made plenty of VitaCubes on the same day, and then each day, we plated one cube. Each day, we respected the very same conditions, every step was timed.<br> | Therefore, we designed a one week experiment: we made plenty of VitaCubes on the same day, and then each day, we plated one cube. Each day, we respected the very same conditions, every step was timed.<br> | ||
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<img src="https://static.igem.org/mediawiki/2015/6/6f/PBScsurvivalrate.jpeg" alt="Graph"> <br> | <img src="https://static.igem.org/mediawiki/2015/6/6f/PBScsurvivalrate.jpeg" alt="Graph"> <br> | ||
<b>Figure 2</b><br> | <b>Figure 2</b><br> | ||
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<div class="column-right" align="justify"> | <div class="column-right" align="justify"> | ||
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<img src="https://static.igem.org/mediawiki/2015/0/0e/PBlactococcussurvival.jpeg" alt="Graph"><br> | <img src="https://static.igem.org/mediawiki/2015/0/0e/PBlactococcussurvival.jpeg" alt="Graph"><br> | ||
<b>Figure 3</b><br> | <b>Figure 3</b><br> | ||
+ | </div> | ||
On graph 2, we can see that the yeasts are very resistant and have a very high survival rate, decreasing very slowly over time. The survival rate is even higher than 1 because yeasts are growing very fast, so in 20 minutes in the water, they divide. In conclusion, the yeasts can be stored more than one week in a VitaCube.<br> | On graph 2, we can see that the yeasts are very resistant and have a very high survival rate, decreasing very slowly over time. The survival rate is even higher than 1 because yeasts are growing very fast, so in 20 minutes in the water, they divide. In conclusion, the yeasts can be stored more than one week in a VitaCube.<br> | ||
On graph 3, we can see that <i>L.</i> lactis survival rate is low since the first day and is decreasing very quickly every day. After 4 days of drying, the survival rate started to be very very low. Nevertheless, there are still around 10<sup>5</sup> cells in a VitaCube after one week, each is far enough to make a culture from it.<br> | On graph 3, we can see that <i>L.</i> lactis survival rate is low since the first day and is decreasing very quickly every day. After 4 days of drying, the survival rate started to be very very low. Nevertheless, there are still around 10<sup>5</sup> cells in a VitaCube after one week, each is far enough to make a culture from it.<br> | ||
Looking at the graphs, we can see that the mix of both strains doesn't influence their survival. | Looking at the graphs, we can see that the mix of both strains doesn't influence their survival. | ||
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Revision as of 12:14, 21 October 2015