Difference between revisions of "Team:Paris Bettencourt/Notebook/Manufacturing"

Line 6: Line 6:
 
<b>Monday Jul. 27</b><br>
 
<b>Monday Jul. 27</b><br>
 
Beginning of the manufacturing project! Let's do it!<br>
 
Beginning of the manufacturing project! Let's do it!<br>
−
<br>
+
<ul>
 +
<li>Choice of the strain of yeast: Sc. M Cherry, wich is resistant to geneticin at a concentration of 200µL/mL</li>
 +
</ul> <br>
 +
We will have to calculate a survival rate so we have to be able to know how many cells we have in the first place for each experiment:
 +
<ul>
 +
<li>Centrifuge the culture tube</li>
 +
<li>Throw the media away</li>
 +
<li>Replace it by a known quantity of osmosed water</li>
 +
<li>Take the OD, doing the blank with osmosed water</li>
 +
<li>Make dilutions of the yeasts/water solution to plate them</li>
 +
<li>Deduce the concentration of Sc. M Cherry corresponding to 1 OD</li>
 +
</ul><br>
 +
First try today and plating of the different dilutions on YPD agar+ GEN (200)
 +
 
  
−
<b>Friday, Jul. 24</b>
+
<b>Tuesday, Jul. 28</b>
 +
First idea: drying the yeasts
 
<ul>
 
<ul>
−
<li>Made the project, it is awesome!</li>
+
<li></li>
 +
<li>Centrifuge the culture tube</li>
 +
<li>Throw the media away, replace it with a known quantity of osmosed water, vortex, take the OD</li>
 +
<li>Centrifuge again, throw the water away keeping a very small amount of it to detach the yeasts from the tube easily</li>
 +
<li>Spread on aluminium</li>
 +
<li>Let it dry for 2 hours</li>
 +
<li>Scratch the aluminium to detach the dried yeasts</li>
 
</ul>
 
</ul>
  

Revision as of 11:18, 13 August 2015

Monday Jul. 27
Beginning of the manufacturing project! Let's do it!

  • Choice of the strain of yeast: Sc. M Cherry, wich is resistant to geneticin at a concentration of 200µL/mL

We will have to calculate a survival rate so we have to be able to know how many cells we have in the first place for each experiment:
  • Centrifuge the culture tube
  • Throw the media away
  • Replace it by a known quantity of osmosed water
  • Take the OD, doing the blank with osmosed water
  • Make dilutions of the yeasts/water solution to plate them
  • Deduce the concentration of Sc. M Cherry corresponding to 1 OD

First try today and plating of the different dilutions on YPD agar+ GEN (200) Tuesday, Jul. 28 First idea: drying the yeasts
  • Centrifuge the culture tube
  • Throw the media away, replace it with a known quantity of osmosed water, vortex, take the OD
  • Centrifuge again, throw the water away keeping a very small amount of it to detach the yeasts from the tube easily
  • Spread on aluminium
  • Let it dry for 2 hours
  • Scratch the aluminium to detach the dried yeasts
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