Difference between revisions of "Team:Paris Bettencourt/Notebook/Riboswitch"
Line 77: | Line 77: | ||
<h2>Procedure</h2> | <h2>Procedure</h2> | ||
− | + | First, digestion : | |
<li> PCR1 | <li> PCR1 | ||
ADN : 40uL = 2,4 ug | ADN : 40uL = 2,4 ug | ||
Line 92: | Line 92: | ||
TOT= 128uL</li> | TOT= 128uL</li> | ||
− | <li> 10 min at 37°C</li> | + | <li>10 min at 37°C</li> |
<br> | <br> | ||
− | + | Then PCR purification | |
+ | <li>Nanodrop : nanodrop : PCR 1 = 20,7 ng/uL ;PCR2 = 25,7</li> | ||
<br> | <br> | ||
For the ligation : | For the ligation : |
Revision as of 16:54, 4 September 2015