Difference between revisions of "Team:Paris Bettencourt/Notebook/Riboswitch"
Line 254: | Line 254: | ||
<h2>Goal 2 </h2> | <h2>Goal 2 </h2> | ||
PCR the pSB1C3 backbone and my FINAL riboswitch construction | PCR the pSB1C3 backbone and my FINAL riboswitch construction | ||
+ | |||
+ | <h2>Procedure</h2> | ||
+ | <li>2 PCR each</li> | ||
+ | <li>I1 and I2 using primers 150 and 151</li> | ||
+ | <li>LP1 and LP2 using primers 152 and 153</li> | ||
+ | <li>Results : Gel migration good only for I2 at 1300 bp.</li> | ||
+ | <li>Purification then nano drop : good for I2</li> | ||
+ | |||
+ | <h2>Results</h2> | ||
+ | |||
+ | <div class="column-left">Gel migration : | ||
+ | </div> | ||
+ | |||
+ | <div class="column-right" align="center"><img src="https://static.igem.org/mediawiki/2015/0/09/111222.png" width="350px"> | ||
+ | </div> | ||
+ | <div style="clear:both"></div> | ||
+ | </br></br> |
Revision as of 15:47, 8 September 2015