Difference between revisions of "Team:Paris Bettencourt/Notebook/Riboswitch"
Line 259: | Line 259: | ||
<li>I1 and I2 using primers 150 and 151</li> | <li>I1 and I2 using primers 150 and 151</li> | ||
<li>LP1 and LP2 using primers 152 and 153</li> | <li>LP1 and LP2 using primers 152 and 153</li> | ||
− | <li>Purification then nano drop : good for I2</li> | + | <li>Purification then nano drop : good for I2 : 112.4 ng/uL </li> |
<h2>Results</h2> | <h2>Results</h2> | ||
− | <div class="column-left">Gel migration : < | + | <div class="column-left">Gel migration : <> |
It shows a good length only for I2 at 1300 bp. | It shows a good length only for I2 at 1300 bp. | ||
</div> | </div> | ||
Line 271: | Line 271: | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
</br></br> | </br></br> | ||
+ | |||
+ | <h2>Goal 3 </h2> | ||
+ | Do the PCR again | ||
+ | |||
+ | <h2>Procedure</h2> | ||
+ | <style type="text/css"> | ||
+ | .tg {border-collapse:collapse;border-spacing:0;} | ||
+ | .tg td{font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} | ||
+ | .tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} | ||
+ | .tg .tg-s6z2{text-align:center} | ||
+ | </style> | ||
+ | <table class="tg"> | ||
+ | <tr> | ||
+ | <th class="tg-031e"></th> | ||
+ | <th class="tg-031e">Template</th> | ||
+ | <th class="tg-031e">Primers</th> | ||
+ | <th class="tg-031e">MasterMix</th> | ||
+ | <th class="tg-031e">Water</th> | ||
+ | <th class="tg-031e">TOTAL</th> | ||
+ | <th class="tg-s6z2">Annealing T°C</th> | ||
+ | <th class="tg-031e">Elongation time</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">I3</td> | ||
+ | <td class="tg-031e">2ng = 2 uL</td> | ||
+ | <td class="tg-031e">1 uL</td> | ||
+ | <td class="tg-031e">25 uL</td> | ||
+ | <td class="tg-031e">22 uL</td> | ||
+ | <td class="tg-031e">50uL</td> | ||
+ | <td class="tg-s6z2" rowspan="4">56°C</td> | ||
+ | <td class="tg-031e" rowspan="4">2 min</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">I4</td> | ||
+ | <td class="tg-031e">2ng = 2 uL</td> | ||
+ | <td class="tg-031e">2 uL</td> | ||
+ | <td class="tg-031e">25 uL</td> | ||
+ | <td class="tg-031e">21 uL</td> | ||
+ | <td class="tg-031e">50uL</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">LP3</td> | ||
+ | <td class="tg-031e">25ng = 1uL</td> | ||
+ | <td class="tg-031e">1 uL</td> | ||
+ | <td class="tg-031e">25 uL</td> | ||
+ | <td class="tg-031e">23 uL</td> | ||
+ | <td class="tg-031e">50uL</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">LP4</td> | ||
+ | <td class="tg-031e">25ng = 1uL</td> | ||
+ | <td class="tg-031e">2 uL</td> | ||
+ | <td class="tg-031e">25 uL</td> | ||
+ | <td class="tg-031e">22 uL</td> | ||
+ | <td class="tg-031e">50uL</td> | ||
+ | </tr> | ||
+ | </table> |
Revision as of 16:14, 8 September 2015