Team:Hamburg/Description
Project Overview
We want to establish a biotechnological production of miRNA2911 [1] in E. coli. The production shall furthermore be induced by light, for which we plan to use BioBricks from the Registry like the Cph8-EnvZ system.
After our bacteria have produced enough of the miRNA, we want them to lyse themselves after a heat-induction. We plan on using the GroEL promoter in combination with a lysing gene pezT.
After the bacteria have lysed themselves, the miRNA could in principle be extracted from the solution and applied as a drug against influenza A infections.
In the following section we will give a detailed description of the theoretical background of each component.
- Zhou Z, Li X, Liu J, et al. Honeysuckle-encoded atypical microRNA2911 directly targets influenza A viruses. Cell Res. 2015;25(1):39-49.
- http://www.cdc.gov/media/subtopic/library/diseaseagents/11823s.jpg
- http://lbc.msu.edu/evo-ed/Images/Ecoli/ecoli.png
- https://de.wikipedia.org/wiki/Tee#/media/File:Chinese_tea,_gancha.jpg