Team:Paris Bettencourt/Notebook/Manufacturing

Monday Jul. 27
Beginning of the manufacturing project! Let's do it!

  • Choice of the strain of yeast: Sc. M Cherry, wich is resistant to geneticin at a concentration of 200µL/mL

We will have to calculate a survival rate so we have to be able to know how many cells we have in the first place for each experiment:
  • Centrifuge the culture tube
  • Throw the media away
  • Replace it by a known quantity of osmosed water
  • Take the OD, doing the blank with osmosed water
  • Make dilutions of the yeasts/water solution to plate them
  • Deduce the concentration of Sc. M Cherry corresponding to 1 OD

First try today and plating of the different dilutions on YPD agar+ GEN (200) Tuesday, Jul. 28 First idea: drying the yeasts
  • Centrifuge the culture tube
  • Throw the media away, replace it with a known quantity of osmosed water, vortex, take the OD
  • Centrifuge again, throw the water away keeping a very small amount of it to detach the yeasts from the tube easily
  • Spread on aluminium
  • Let it dry for 2 hours
  • Scratch the aluminium to detach the dried yeasts
eaeauieuaie

eiuaeuaie