Team:Paris Bettencourt/Notebook/Manufacturing
Ferment It Yourself
iGEM Paris-Bettencourt 2O15
- Background
- Design
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Notebook
Vitamin A Vitamin B2 Vitamin B12 Phytase Riboswitch Differentiation on E. coli Differentiation on S. cerevisiae Manufacturing Idli and Micro-organisms Monday Jul. 27
Beginning of the manufacturing project! Let's do it!
- Choice of the strain of yeast: Sc. M Cherry, wich is resistant to geneticin at a concentration of 200µL/mL
We will have to calculate a survival rate so we have to be able to know how many cells we have in the first place for each experiment:- Centrifuge the culture tube
- Throw the media away
- Replace it by a known quantity of osmosed water
- Take the OD, doing the blank with osmosed water
- Make dilutions of the yeasts/water solution to plate them
- Deduce the concentration of Sc. M Cherry corresponding to 1 OD
First try today and plating of the different dilutions on YPD agar+ GEN (200) Tuesday, Jul. 28 First idea: drying the yeasts- Centrifuge the culture tube
- Throw the media away, replace it with a known quantity of osmosed water, vortex, take the OD
- Centrifuge again, throw the water away keeping a very small amount of it to detach the yeasts from the tube easily
- Spread on aluminium
- Let it dry for 2 hours
- Scratch the aluminium to detach the dried yeasts
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