Exeter iGEM Lab Book

Welcome to our lab book. The following pages contain a chronological description of everything we did in the lab – starting on Day 1. Different days contain short descriptions of our experiments and notes, as well as references to the appropriate protocols.

We were not adept at keeping a concise lab book during the first few weeks of iGEM – the descriptions get more organised and detailed as the weeks progress. The first part of our lab book relates to the Interlab Study, and the later parts to our actual project, Ribonostics.

All protocols can be found at the bottom of the page,and there will links throughout. If you have any questions you can email the team or individual members emails can be found on our team page.

04/06/15

Interlab Study - hydrating DNA, transformation into competent DH5α cells, overnight cultures, mini prep.

05/06/15

Digestion of our insert and backbone.

Results of our transformations from our plates.

08/06/15

Diagnostic digestion of previous ligations:

II3504 + J23108 = iGEMILS08A

Il3504 + J23108 = iGEMILS08B

Used EcoRI and SpeI during our digestion.

09/06/15

Diagnostic digestion of previous ligations.

Planned to miniprep iGEMILS08A AND iGEMILS08B, BUT we realised that we were using the wrong BioBricks all along - J23111, J23109, and J23108, INSTEAD of J23101, J23106 and J23117.

Resuspending DNA from Kit 1 (BioBricks J23101, J23106, J23117).

Hydrating DNA from the kit.

Poured plates necessary for our experiments.