Difference between revisions of "Team:Paris Bettencourt/Protocols/Yeast lysis with NaOH"
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| − | + | <ul> | |
| + | <li> 20 µl NaOH into PCR tubes | ||
| + | <li> Pick colonies into NaOH | ||
| + | <li> Incubate at 95°C for ~45 minutes | ||
| + | <li> Centrifugate at 8000 krpm for 10 minutes | ||
| + | <li> Use 1 µl supernatant as template in a (10 µl) PCR | ||
| + | </ul> | ||
</ul> | </ul> | ||
Latest revision as of 15:18, 20 August 2015