Team:Exeter/Protocols
Protocols
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- E.coli transformation.
- gBlock Prep. and HiFi assembly.
- Competent cells preparation.
- Protein extraction (BugBuster).
- LB agar broth recipe.
- Agarose gel recipe.
- Running gel electrophoresis.
- Using the autoclave.
- DNA restriction digest.
- Thermo scientific FastDigest.
- DNA gel extraction.
- Cell glycerol stocks.
- Promega DNA ligation.
- Qiagen DNA miniprep.
- Liquid cell cultures.
- MgCl2 and CaCl2 recipes.
- Sterile 40% glycerol recipe.
- Qubit for DNA concentration.
- Dried DNA rehydration.
- Promega S30 cell free kit.
- Solid agar recipe for agar plates.