Team:Paris Bettencourt/Protocols/Heat Shock transformation
Ferment It Yourself
iGEM Paris-Bettencourt 2O15
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Notebook
Vitamin A Vitamin B2 Vitamin B12 Phytase Riboswitch Differentiation on E. coli Differentiation on S. cerevisiae Manufacturing Idli and Micro-organisms Heat Shock transformation protocol
- Thaw frozen chemically competent cells (20µL aliquots) on ice for 10min.
- add 2µL of ligation product (or 0.5µL of miniprep) and incubate the cells 30sec at 42°C.
- put the cells back on ice for 2min.
- add 200µL of LB to the cells and incubate 2 hours at 37°C.
- plate the cells on LB + erythromycin (150µg/mL) or LB + erythromycin (10µg/mL), incubation at 37°C overnight.