Team:Paris Bettencourt/Protocols/Ligation

Ferment It Yourself

iGEM Paris-Bettencourt 2O15

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Notebook

Vitamin A	Vitamin B2	Vitamin B12
Phytase	Riboswitch	Differentiation on E. coli
Differentiation on S. cerevisiae	Manufacturing	Idli and Micro-organisms

Ligation Protocol Mix the following vector 100ng insert 300ng 2µL T4 DNA ligase buffer 10X 1µL T4 DNA ligase up to 20µL of water incubate 10 to 15 minutes at 22°C incubate 5 minutes at 70°C