Difference between revisions of "Team:Paris Bettencourt/Notebook/Phytase"

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5’Primer  of Kan resistance gene with tails use to transformation with the PHO85 gene of the yeast.<br>
 
5’Primer  of Kan resistance gene with tails use to transformation with the PHO85 gene of the yeast.<br>
<br>5’-<span style="color:#9A1717">TATCATTATATATACATGGCTACGGTTTTTCGCTGACGGGCTGCGATAATCATTTGCA
+
<br>5’-<span style="color:#9A1717">TATCATTATATATACATGGCTACGGTTTTTCGCTGACGGGCTGCG<span style="color:#179A89>ATAATCATTTGCA
<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;tail homologie PHO85
+
<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;tail <span style="color:#9A1717">homologie PHO85
  
<br>TCCATACATTTTGATGGC -3’
+
<br><span style="color:#179A89>TCCATACATTTTGATGGC -3’
 
<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Primer Kan
 
<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Primer Kan
 
<br>
 
<br>

Revision as of 13:59, 14 August 2015

Background

Aims

Results

Cobalamin (vitamin B12) deficiency is widely spread in India, due to diet that is mostly vegetarian. We aimed at introducing a high-level cobalamin producer to the rice batter. Tadaa.

5/08/15


Design primers

5’Primer of Kan resistance gene with tails use to transformation with the PHO85 gene of the yeast.

5’-TATCATTATATATACATGGCTACGGTTTTTCGCTGACGGGCTGCGhomologie PHO85
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