Difference between revisions of "Team:Paris Bettencourt/jb"

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<html><head><title>Untitled document</title><meta content="text/html; charset=UTF-8" http-equiv="content-type"><style type="text/css">ol{margin:0;padding:0}.c0{border-right-style:solid;padding:2pt 2pt 2pt 2pt;border-bottom-color:#000000;border-top-width:1pt;border-right-width:1pt;border-left-color:#000000;vertical-align:bottom;border-right-color:#000000;border-left-width:1pt;border-top-style:solid;border-left-style:solid;border-bottom-width:1pt;width:348pt;border-top-color:#000000;border-bottom-style:solid}.c1{border-right-style:solid;padding:2pt 2pt 2pt 2pt;border-bottom-color:#000000;border-top-width:1pt;border-right-width:1pt;border-left-color:#000000;vertical-align:bottom;border-right-color:#000000;border-left-width:1pt;border-top-style:solid;border-left-style:solid;border-bottom-width:1pt;width:245.2pt;border-top-color:#000000;border-bottom-style:solid}.c3{border-right-style:solid;padding:2pt 2pt 2pt 2pt;border-bottom-color:#000000;border-top-width:1pt;border-right-width:1pt;border-left-color:#000000;vertical-align:bottom;border-right-color:#000000;border-left-width:1pt;border-top-style:solid;border-left-style:solid;border-bottom-width:1pt;width:54.8pt;border-top-color:#000000;border-bottom-style:solid}.c2{color:#000000;font-weight:normal;text-decoration:none;vertical-align:baseline;font-size:10pt;font-family:"Arial";font-style:normal}.c4{padding-top:0pt;padding-bottom:0pt;line-height:1.0;text-align:left;direction:ltr}.c6{orphans:2;widows:2;direction:ltr;height:11pt}.c8{background-color:#ffffff;max-width:648pt;padding:72pt 72pt 72pt 72pt}.c7{border-collapse:collapse;margin-right:auto}.c5{height:0pt}.title{padding-top:0pt;color:#000000;font-size:21pt;padding-bottom:0pt;font-family:"Trebuchet MS";line-height:1.15;page-break-after:avoid;orphans:2;widows:2;text-align:left}.subtitle{padding-top:0pt;color:#666666;font-size:13pt;padding-bottom:10pt;font-family:"Trebuchet MS";line-height:1.15;page-break-after:avoid;font-style:italic;orphans:2;widows:2;text-align:left}li{color:#000000;font-size:11pt;font-family:"Arial"}p{margin:0;color:#000000;font-size:11pt;font-family:"Arial"}h1{padding-top:10pt;color:#000000;font-size:16pt;padding-bottom:0pt;font-family:"Trebuchet MS";line-height:1.15;page-break-after:avoid;orphans:2;widows:2;text-align:left}h2{padding-top:10pt;color:#000000;font-weight:bold;font-size:13pt;padding-bottom:0pt;font-family:"Trebuchet MS";line-height:1.15;page-break-after:avoid;orphans:2;widows:2;text-align:left}h3{padding-top:8pt;color:#666666;font-weight:bold;font-size:12pt;padding-bottom:0pt;font-family:"Trebuchet MS";line-height:1.15;page-break-after:avoid;orphans:2;widows:2;text-align:left}h4{padding-top:8pt;color:#666666;text-decoration:underline;font-size:11pt;padding-bottom:0pt;font-family:"Trebuchet MS";line-height:1.15;page-break-after:avoid;orphans:2;widows:2;text-align:left}h5{padding-top:8pt;color:#666666;font-size:11pt;padding-bottom:0pt;font-family:"Trebuchet MS";line-height:1.15;page-break-after:avoid;orphans:2;widows:2;text-align:left}h6{padding-top:8pt;color:#666666;font-size:11pt;padding-bottom:0pt;font-family:"Trebuchet MS";line-height:1.15;page-break-after:avoid;font-style:italic;orphans:2;widows:2;text-align:left}</style></head><body class="c8"><p class="c6"><span></span></p><a href="#" name="2bb38ce7bdbdb94f4a6ddd41c742ce0de007afe3"></a><a href="#" name="0"></a><table cellpadding="0" cellspacing="0" class="c7"><tbody><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.053</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">HO-Poly-KanMX4-HO amplification (reverse) + tail</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">TGAAATGGCGAGTATTGATAATGATAAACTCTGTCAAGGAGGGTATTCTGGGC</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.056</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">gBlock2 amplification (forward) + tail</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">TCACAACAAGAATTTTGCAGAGGACCTAACCGAAGGAAAGTTTTCCTTCCCAACAATC</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.057</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">gBlock2 amplification (reverse) + tail</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">GGACTTGATAGAATTTTGTCTGGTTGACTCGGATGCAATGGAGGACCGAACAAT</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.058</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">gBlock3 amplification (forward)</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">AACGTTATTGTTCGGTCCTCCATTGC</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.059</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">gBlock3 amplification (reverse) + tail</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">GTCCAGATTGACTCGAATGGGTGTAATGCCAGTATTTGACCAATGAATTGTAGCCTCAAG</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.060</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">gBlock4 amplification (forward)</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">CTTGAGGCTACAATTCATTGGTCAAATAC</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.061</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">gBlock4 amplification (reverse) + tail</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">TGTACGGGCGACAGTCACATCATGCCCCTGCAGGGTACCGCGAATTCCCC</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.117</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">forward primer for gblock 1 part 1</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">CGTCCAAGACATACTGCGTTTACG</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.118</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">reverse primer for gblock 1 part 1</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">GTGTGCAACATTCCCACTACATTTTGAATG</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.119</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">forward primer for gblock 1 part 2</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">CATTCAAAATGTAGTGGGAATGTTGCAC</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.120</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">reverse primer for gblock 1 part 2</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">TGGATTGTTGGGAAGGAAAACTTTCCTT</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.121</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">forward primer gblock 1 + restriction site ecor1</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">TATACTGCAGTCTAGAACTAGTGGATCCATGGATTAC</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.122</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">reverse primer gblock 1 + tail</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">AGCCTGGAGGAAGGGTTAGCATGGATGGAATGGATTGTTGGGAAGGAAAACTTTCCTT</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.123</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">forward primer TDH3 from registry with HO binding tail</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">GCCCAGAATACCCTCCTTGACAGACAGTTTATTCCTGGCATCCACTAAATATAATG</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.124</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">reverse primer TDH3 from registry with RE binding site</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">TATACTGCAGTTTGTTTGTTTATGTGTGTTTATTCGAAACTAAGTTC</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.125</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">forward primer full gblock 1 with new promoter</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">GCCCAGAATACCCTCCTTGACAG</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.126</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">reverse primer full gblock 1 with new promoter</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">CATGCTAACCCTTCCTCCAGGCT</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.127</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">forward primer gblock 2 + tail</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">TCACAACAAGAATTTTGCAGAGGACCTAACCGAAGGAAAGTTTTCCTTCCCAACAATC</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.128</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">reverse primer gblock 2 + tail</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">GGACTTGATAGAATTTTGTCTGGTTGACTCGGATGCAATGGAGGACCGAACAAT</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.129</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">forward primer gblock 3</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">AACGTTATTGTTCGGTCCTCCATTGC</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.130</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">reverse primer gblock 3 + tail</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">GTCCAGATTGACTCGAATGGGTGTAATGCCAGTATTTGACCAATGAATTGTAGCCTCAAG</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.131</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">forward primer gblock 4</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">CTTGAGGCTACAATTCATTGGTCAAATAC</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.132</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">reverse primer gblock 4 HO tail</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">TGTACGGGCGACAGTCACATCATGCCCCTGCAGGGTACCGCGAATTCCCC</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.133</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">reverse primer gblock 4 HMG tail</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">CATGCGCCTATTATTACTTCAATAGATGGCAGGGTACCGCGAATTCCCC</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.134</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">forward primer vector HO with HMG</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">GCTTTAATTTGCGGCCGGTACCCAATTCGCCAGGGGCATGATGTGACTGTCG</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.135</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">forward primer vector HO without HMG (binds to gBlock4)</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">TCGCTATACTGGGGAATTCGCGGTACCCTGCAGGGGCATGATGTGACTGTCG</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.136</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">reverse primer vector HO (binds to TDH3?)</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">TATATTTAGTGGATGCCAGGAATAAACTGTCTGTCAAGGAGGGTATTCTGGGC</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.137</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">formard primer HMG+tail with RE</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">TATATCTAGACCCGCCGCCACCATGCAAAG</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.138</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">reverse primer HMG+tail with restriction site</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">TATACTCGAGTCACTGTTGAGACCTCAAATCCTGTAAG</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.139</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">forward primer promoter HMG terminator</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">TCGCTATACTGGGGAATTCGCGGTACCCTGCCATCTATTGAAGTAATAATAGGCGCATG</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.140</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">reverse primer HMG + tail HO</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">TGTACGGGCGACAGTCACATCATGCCCCTGGCGAATTGGGTACCGGCCG</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.141</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">reverse primer gblock 1 part 1 (binds to part2))</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">TCCTCAACGTCGTCCATCAGTAAGCTCGCTGTGTGCAACATTCCCACTACATTTTGAATG</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.142</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">forward primer gblock 1 + tail that binds to HO pl</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">GCCCAGAATACCCTCCTTGACAGCGTCCAAGACATACTGCGTTTACG</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.143</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">reverse primer HO + tail crappy ADH1</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">TCGACACGTAAACGCAGTATGTCTTGGACGCTGTCAAGGAGGGTATTCTGGGC</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.144</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">forward primer gblock 1 + tail TDH3</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">GTTTCGAATAAACACACATAAACAAACAAATCTAGAACTAGTGGATCCATGGATTAC</span></p></td></tr><tr class="c5"><td class="c3" colspan="1" rowspan="1"><p class="c4"><span class="c2">o15.145</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c4"><span class="c2">reverse primer TDH3 + tail for kozac</span></p></td><td class="c0" colspan="1" rowspan="1"><p class="c4"><span class="c2">TCCATGGATCCACTAGTTCTAGATTTGTTTGTTTATGTGTGTTTATTCGAAACTAAGTTC</span></p></td></tr></tbody></table><p class="c6"><span></span></p></body></html>
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<html>
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<p class="c6">
 +
<span></span>
 +
</p>
 +
<a href="#" name="2bb38ce7bdbdb94f4a6ddd41c742ce0de007afe3"></a><a href="#" name="0"></a>
 +
<table cellpadding="0" cellspacing="0" class="c7">
 +
<tbody>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.053</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">HO-Poly-KanMX4-HO amplification (reverse) + tail</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TGAAATGGCGAGTATTGATAATGATAAACTCTGTCAAGGAGGGTATTCTGGGC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.056</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">gBlock2 amplification (forward) + tail</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TCACAACAAGAATTTTGCAGAGGACCTAACCGAAGGAAAGTTTTCCTTCCCAACAATC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.057</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">gBlock2 amplification (reverse) + tail</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">GGACTTGATAGAATTTTGTCTGGTTGACTCGGATGCAATGGAGGACCGAACAAT</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.058</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">gBlock3 amplification (forward)</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">AACGTTATTGTTCGGTCCTCCATTGC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.059</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">gBlock3 amplification (reverse) + tail</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">GTCCAGATTGACTCGAATGGGTGTAATGCCAGTATTTGACCAATGAATTGTAGCCTCAAG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.060</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">gBlock4 amplification (forward)</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">CTTGAGGCTACAATTCATTGGTCAAATAC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.061</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">gBlock4 amplification (reverse) + tail</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TGTACGGGCGACAGTCACATCATGCCCCTGCAGGGTACCGCGAATTCCCC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.117</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer for gblock 1 part 1</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">CGTCCAAGACATACTGCGTTTACG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.118</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer for gblock 1 part 1</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">GTGTGCAACATTCCCACTACATTTTGAATG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.119</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer for gblock 1 part 2</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">CATTCAAAATGTAGTGGGAATGTTGCAC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.120</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer for gblock 1 part 2</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TGGATTGTTGGGAAGGAAAACTTTCCTT</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.121</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer gblock 1 + restriction site ecor1</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TATACTGCAGTCTAGAACTAGTGGATCCATGGATTAC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.122</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer gblock 1 + tail</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">AGCCTGGAGGAAGGGTTAGCATGGATGGAATGGATTGTTGGGAAGGAAAACTTTCCTT</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.123</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer TDH3 from registry with HO binding tail</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">GCCCAGAATACCCTCCTTGACAGACAGTTTATTCCTGGCATCCACTAAATATAATG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.124</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer TDH3 from registry with RE binding site</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TATACTGCAGTTTGTTTGTTTATGTGTGTTTATTCGAAACTAAGTTC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.125</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer full gblock 1 with new promoter</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">GCCCAGAATACCCTCCTTGACAG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.126</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer full gblock 1 with new promoter</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">CATGCTAACCCTTCCTCCAGGCT</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.127</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer gblock 2 + tail</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TCACAACAAGAATTTTGCAGAGGACCTAACCGAAGGAAAGTTTTCCTTCCCAACAATC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.128</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer gblock 2 + tail</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">GGACTTGATAGAATTTTGTCTGGTTGACTCGGATGCAATGGAGGACCGAACAAT</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.129</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer gblock 3</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">AACGTTATTGTTCGGTCCTCCATTGC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.130</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer gblock 3 + tail</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">GTCCAGATTGACTCGAATGGGTGTAATGCCAGTATTTGACCAATGAATTGTAGCCTCAAG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.131</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer gblock 4</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">CTTGAGGCTACAATTCATTGGTCAAATAC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.132</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer gblock 4 HO tail</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TGTACGGGCGACAGTCACATCATGCCCCTGCAGGGTACCGCGAATTCCCC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.133</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer gblock 4 HMG tail</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">CATGCGCCTATTATTACTTCAATAGATGGCAGGGTACCGCGAATTCCCC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.134</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer vector HO with HMG</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">GCTTTAATTTGCGGCCGGTACCCAATTCGCCAGGGGCATGATGTGACTGTCG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.135</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer vector HO without HMG (binds to gBlock4)</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TCGCTATACTGGGGAATTCGCGGTACCCTGCAGGGGCATGATGTGACTGTCG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.136</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer vector HO (binds to TDH3?)</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TATATTTAGTGGATGCCAGGAATAAACTGTCTGTCAAGGAGGGTATTCTGGGC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.137</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">formard primer HMG+tail with RE</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TATATCTAGACCCGCCGCCACCATGCAAAG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.138</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer HMG+tail with restriction site</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TATACTCGAGTCACTGTTGAGACCTCAAATCCTGTAAG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.139</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer promoter HMG terminator</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TCGCTATACTGGGGAATTCGCGGTACCCTGCCATCTATTGAAGTAATAATAGGCGCATG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.140</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer HMG + tail HO</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TGTACGGGCGACAGTCACATCATGCCCCTGGCGAATTGGGTACCGGCCG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.141</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer gblock 1 part 1 (binds to part2))</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TCCTCAACGTCGTCCATCAGTAAGCTCGCTGTGTGCAACATTCCCACTACATTTTGAATG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.142</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer gblock 1 + tail that binds to HO pl</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">GCCCAGAATACCCTCCTTGACAGCGTCCAAGACATACTGCGTTTACG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.143</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer HO + tail crappy ADH1</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TCGACACGTAAACGCAGTATGTCTTGGACGCTGTCAAGGAGGGTATTCTGGGC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.144</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer gblock 1 + tail TDH3</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">GTTTCGAATAAACACACATAAACAAACAAATCTAGAACTAGTGGATCCATGGATTAC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.145</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer TDH3 + tail for kozac</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TCCATGGATCCACTAGTTCTAGATTTGTTTGTTTATGTGTGTTTATTCGAAACTAAGTTC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
</tbody>
 +
</table>
 +
<p class="c6">
 +
<span></span>
 +
</p>
 +
</html>
 +
 
 +
{{Paris_Bettencourt/footer}}

Revision as of 15:30, 14 August 2015


o15.053

HO-Poly-KanMX4-HO amplification (reverse) + tail

TGAAATGGCGAGTATTGATAATGATAAACTCTGTCAAGGAGGGTATTCTGGGC

o15.056

gBlock2 amplification (forward) + tail

TCACAACAAGAATTTTGCAGAGGACCTAACCGAAGGAAAGTTTTCCTTCCCAACAATC

o15.057

gBlock2 amplification (reverse) + tail

GGACTTGATAGAATTTTGTCTGGTTGACTCGGATGCAATGGAGGACCGAACAAT

o15.058

gBlock3 amplification (forward)

AACGTTATTGTTCGGTCCTCCATTGC

o15.059

gBlock3 amplification (reverse) + tail

GTCCAGATTGACTCGAATGGGTGTAATGCCAGTATTTGACCAATGAATTGTAGCCTCAAG

o15.060

gBlock4 amplification (forward)

CTTGAGGCTACAATTCATTGGTCAAATAC

o15.061

gBlock4 amplification (reverse) + tail

TGTACGGGCGACAGTCACATCATGCCCCTGCAGGGTACCGCGAATTCCCC

o15.117

forward primer for gblock 1 part 1

CGTCCAAGACATACTGCGTTTACG

o15.118

reverse primer for gblock 1 part 1

GTGTGCAACATTCCCACTACATTTTGAATG

o15.119

forward primer for gblock 1 part 2

CATTCAAAATGTAGTGGGAATGTTGCAC

o15.120

reverse primer for gblock 1 part 2

TGGATTGTTGGGAAGGAAAACTTTCCTT

o15.121

forward primer gblock 1 + restriction site ecor1

TATACTGCAGTCTAGAACTAGTGGATCCATGGATTAC

o15.122

reverse primer gblock 1 + tail

AGCCTGGAGGAAGGGTTAGCATGGATGGAATGGATTGTTGGGAAGGAAAACTTTCCTT

o15.123

forward primer TDH3 from registry with HO binding tail

GCCCAGAATACCCTCCTTGACAGACAGTTTATTCCTGGCATCCACTAAATATAATG

o15.124

reverse primer TDH3 from registry with RE binding site

TATACTGCAGTTTGTTTGTTTATGTGTGTTTATTCGAAACTAAGTTC

o15.125

forward primer full gblock 1 with new promoter

GCCCAGAATACCCTCCTTGACAG

o15.126

reverse primer full gblock 1 with new promoter

CATGCTAACCCTTCCTCCAGGCT

o15.127

forward primer gblock 2 + tail

TCACAACAAGAATTTTGCAGAGGACCTAACCGAAGGAAAGTTTTCCTTCCCAACAATC

o15.128

reverse primer gblock 2 + tail

GGACTTGATAGAATTTTGTCTGGTTGACTCGGATGCAATGGAGGACCGAACAAT

o15.129

forward primer gblock 3

AACGTTATTGTTCGGTCCTCCATTGC

o15.130

reverse primer gblock 3 + tail

GTCCAGATTGACTCGAATGGGTGTAATGCCAGTATTTGACCAATGAATTGTAGCCTCAAG

o15.131

forward primer gblock 4

CTTGAGGCTACAATTCATTGGTCAAATAC

o15.132

reverse primer gblock 4 HO tail

TGTACGGGCGACAGTCACATCATGCCCCTGCAGGGTACCGCGAATTCCCC

o15.133

reverse primer gblock 4 HMG tail

CATGCGCCTATTATTACTTCAATAGATGGCAGGGTACCGCGAATTCCCC

o15.134

forward primer vector HO with HMG

GCTTTAATTTGCGGCCGGTACCCAATTCGCCAGGGGCATGATGTGACTGTCG

o15.135

forward primer vector HO without HMG (binds to gBlock4)

TCGCTATACTGGGGAATTCGCGGTACCCTGCAGGGGCATGATGTGACTGTCG

o15.136

reverse primer vector HO (binds to TDH3?)

TATATTTAGTGGATGCCAGGAATAAACTGTCTGTCAAGGAGGGTATTCTGGGC

o15.137

formard primer HMG+tail with RE

TATATCTAGACCCGCCGCCACCATGCAAAG

o15.138

reverse primer HMG+tail with restriction site

TATACTCGAGTCACTGTTGAGACCTCAAATCCTGTAAG

o15.139

forward primer promoter HMG terminator

TCGCTATACTGGGGAATTCGCGGTACCCTGCCATCTATTGAAGTAATAATAGGCGCATG

o15.140

reverse primer HMG + tail HO

TGTACGGGCGACAGTCACATCATGCCCCTGGCGAATTGGGTACCGGCCG

o15.141

reverse primer gblock 1 part 1 (binds to part2))

TCCTCAACGTCGTCCATCAGTAAGCTCGCTGTGTGCAACATTCCCACTACATTTTGAATG

o15.142

forward primer gblock 1 + tail that binds to HO pl

GCCCAGAATACCCTCCTTGACAGCGTCCAAGACATACTGCGTTTACG

o15.143

reverse primer HO + tail crappy ADH1

TCGACACGTAAACGCAGTATGTCTTGGACGCTGTCAAGGAGGGTATTCTGGGC

o15.144

forward primer gblock 1 + tail TDH3

GTTTCGAATAAACACACATAAACAAACAAATCTAGAACTAGTGGATCCATGGATTAC

o15.145

reverse primer TDH3 + tail for kozac

TCCATGGATCCACTAGTTCTAGATTTGTTTGTTTATGTGTGTTTATTCGAAACTAAGTTC