Difference between revisions of "Team:Paris Bettencourt/jb"

 
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{{Template:Paris_Bettencourt}}
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{{Paris_Bettencourt/header}}
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{{Paris_Bettencourt/menu}}
 +
{{Paris_Bettencourt/notebookBanner}}
 +
 
 +
 
 
<html>
 
<html>
−
<h1>Vitamine A deficiency</h1>
+
<hr>
−
<br></br>
+
<p class="c6">
−
Vitamine A deficiency is common in poor countries but rarely observed in developed countries.
+
<span></span>
−
<br></br>
+
</p>
−
<h2>Cause</h2>
+
<a href="#" name="2bb38ce7bdbdb94f4a6ddd41c742ce0de007afe3"></a><a href="#" name="0"></a>
−
Common symptoms of VAD are visual loss (night blindness and xerophtalmia), impaired immune function, cancer and birth defects.
+
<table cellpadding="0" cellspacing="0" class="c7">
−
<br></br>
+
<tbody>
−
<h2>Symptoms</h2>
+
<tr class="c5">
−
It is caused by insufficient consumption of vitamin A and iron deprivation.
+
<td class="c3" colspan="1" rowspan="1">
−
<br></br>
+
<p class="c4">
−
<h2>Prevalence</h2>
+
<span class="c2">o15.053</span>
−
between 250,000 and 500,000 children are estimated going blind each year because of a vitamin A deficiency every year.
+
</p>
−
It increases maternal mortality and decrease the efficiency of lactation.
+
</td>
−
<br></br>
+
<td class="c1" colspan="1" rowspan="1">
−
<h2>Fortification attempt</h2>
+
<p class="c4">
−
VAD is also increasing the severity and the susceptibility to infections
+
<span class="c2">HO-Poly-KanMX4-HO amplification (reverse) + tail</span>
−
<br></br>
+
</p>
−
according to the WHO report " Global prevalence of vitamin A deficiency in populations at risk"
+
</td>
−
<h2>Link with our project</h2>
+
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TGAAATGGCGAGTATTGATAATGATAAACTCTGTCAAGGAGGGTATTCTGGGC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.056</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">gBlock2 amplification (forward) + tail</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TCACAACAAGAATTTTGCAGAGGACCTAACCGAAGGAAAGTTTTCCTTCCCAACAATC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.057</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">gBlock2 amplification (reverse) + tail</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">GGACTTGATAGAATTTTGTCTGGTTGACTCGGATGCAATGGAGGACCGAACAAT</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.058</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">gBlock3 amplification (forward)</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">AACGTTATTGTTCGGTCCTCCATTGC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.059</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">gBlock3 amplification (reverse) + tail</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">GTCCAGATTGACTCGAATGGGTGTAATGCCAGTATTTGACCAATGAATTGTAGCCTCAAG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.060</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">gBlock4 amplification (forward)</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">CTTGAGGCTACAATTCATTGGTCAAATAC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.061</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">gBlock4 amplification (reverse) + tail</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TGTACGGGCGACAGTCACATCATGCCCCTGCAGGGTACCGCGAATTCCCC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.117</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer for gblock 1 part 1</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">CGTCCAAGACATACTGCGTTTACG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.118</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer for gblock 1 part 1</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">GTGTGCAACATTCCCACTACATTTTGAATG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.119</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer for gblock 1 part 2</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">CATTCAAAATGTAGTGGGAATGTTGCAC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.120</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer for gblock 1 part 2</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TGGATTGTTGGGAAGGAAAACTTTCCTT</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.121</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer gblock 1 + restriction site ecor1</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TATACTGCAGTCTAGAACTAGTGGATCCATGGATTAC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.122</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer gblock 1 + tail</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">AGCCTGGAGGAAGGGTTAGCATGGATGGAATGGATTGTTGGGAAGGAAAACTTTCCTT</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.123</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer TDH3 from registry with HO binding tail</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">GCCCAGAATACCCTCCTTGACAGACAGTTTATTCCTGGCATCCACTAAATATAATG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.124</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer TDH3 from registry with RE binding site</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TATACTGCAGTTTGTTTGTTTATGTGTGTTTATTCGAAACTAAGTTC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.125</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer full gblock 1 with new promoter</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">GCCCAGAATACCCTCCTTGACAG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.126</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer full gblock 1 with new promoter</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">CATGCTAACCCTTCCTCCAGGCT</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.127</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer gblock 2 + tail</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TCACAACAAGAATTTTGCAGAGGACCTAACCGAAGGAAAGTTTTCCTTCCCAACAATC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.128</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer gblock 2 + tail</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">GGACTTGATAGAATTTTGTCTGGTTGACTCGGATGCAATGGAGGACCGAACAAT</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.129</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer gblock 3</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">AACGTTATTGTTCGGTCCTCCATTGC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.130</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer gblock 3 + tail</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">GTCCAGATTGACTCGAATGGGTGTAATGCCAGTATTTGACCAATGAATTGTAGCCTCAAG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.131</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer gblock 4</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">CTTGAGGCTACAATTCATTGGTCAAATAC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.132</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer gblock 4 HO tail</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TGTACGGGCGACAGTCACATCATGCCCCTGCAGGGTACCGCGAATTCCCC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.133</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer gblock 4 HMG tail</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">CATGCGCCTATTATTACTTCAATAGATGGCAGGGTACCGCGAATTCCCC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.134</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer vector HO with HMG</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">GCTTTAATTTGCGGCCGGTACCCAATTCGCCAGGGGCATGATGTGACTGTCG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.135</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer vector HO without HMG (binds to gBlock4)</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TCGCTATACTGGGGAATTCGCGGTACCCTGCAGGGGCATGATGTGACTGTCG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.136</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer vector HO (binds to TDH3?)</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TATATTTAGTGGATGCCAGGAATAAACTGTCTGTCAAGGAGGGTATTCTGGGC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.137</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">formard primer HMG+tail with RE</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TATATCTAGACCCGCCGCCACCATGCAAAG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.138</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer HMG+tail with restriction site</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TATACTCGAGTCACTGTTGAGACCTCAAATCCTGTAAG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.139</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer promoter HMG terminator</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TCGCTATACTGGGGAATTCGCGGTACCCTGCCATCTATTGAAGTAATAATAGGCGCATG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.140</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer HMG + tail HO</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TGTACGGGCGACAGTCACATCATGCCCCTGGCGAATTGGGTACCGGCCG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.141</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer gblock 1 part 1 (binds to part2))</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TCCTCAACGTCGTCCATCAGTAAGCTCGCTGTGTGCAACATTCCCACTACATTTTGAATG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.142</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer gblock 1 + tail that binds to HO pl</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">GCCCAGAATACCCTCCTTGACAGCGTCCAAGACATACTGCGTTTACG</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.143</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer HO + tail crappy ADH1</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TCGACACGTAAACGCAGTATGTCTTGGACGCTGTCAAGGAGGGTATTCTGGGC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.144</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">forward primer gblock 1 + tail TDH3</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">GTTTCGAATAAACACACATAAACAAACAAATCTAGAACTAGTGGATCCATGGATTAC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
<tr class="c5">
 +
<td class="c3" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">o15.145</span>
 +
</p>
 +
</td>
 +
<td class="c1" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">reverse primer TDH3 + tail for kozac</span>
 +
</p>
 +
</td>
 +
<td class="c0" colspan="1" rowspan="1">
 +
<p class="c4">
 +
<span class="c2">TCCATGGATCCACTAGTTCTAGATTTGTTTGTTTATGTGTGTTTATTCGAAACTAAGTTC</span>
 +
</p>
 +
</td>
 +
</tr>
 +
</tbody>
 +
</table>
 +
<p class="c6">
 +
<span></span>
 +
</p>
 +
<hr>
 
</html>
 
</html>
 +
 +
{{Paris_Bettencourt/footer}}

Latest revision as of 15:31, 14 August 2015


o15.053

HO-Poly-KanMX4-HO amplification (reverse) + tail

TGAAATGGCGAGTATTGATAATGATAAACTCTGTCAAGGAGGGTATTCTGGGC

o15.056

gBlock2 amplification (forward) + tail

TCACAACAAGAATTTTGCAGAGGACCTAACCGAAGGAAAGTTTTCCTTCCCAACAATC

o15.057

gBlock2 amplification (reverse) + tail

GGACTTGATAGAATTTTGTCTGGTTGACTCGGATGCAATGGAGGACCGAACAAT

o15.058

gBlock3 amplification (forward)

AACGTTATTGTTCGGTCCTCCATTGC

o15.059

gBlock3 amplification (reverse) + tail

GTCCAGATTGACTCGAATGGGTGTAATGCCAGTATTTGACCAATGAATTGTAGCCTCAAG

o15.060

gBlock4 amplification (forward)

CTTGAGGCTACAATTCATTGGTCAAATAC

o15.061

gBlock4 amplification (reverse) + tail

TGTACGGGCGACAGTCACATCATGCCCCTGCAGGGTACCGCGAATTCCCC

o15.117

forward primer for gblock 1 part 1

CGTCCAAGACATACTGCGTTTACG

o15.118

reverse primer for gblock 1 part 1

GTGTGCAACATTCCCACTACATTTTGAATG

o15.119

forward primer for gblock 1 part 2

CATTCAAAATGTAGTGGGAATGTTGCAC

o15.120

reverse primer for gblock 1 part 2

TGGATTGTTGGGAAGGAAAACTTTCCTT

o15.121

forward primer gblock 1 + restriction site ecor1

TATACTGCAGTCTAGAACTAGTGGATCCATGGATTAC

o15.122

reverse primer gblock 1 + tail

AGCCTGGAGGAAGGGTTAGCATGGATGGAATGGATTGTTGGGAAGGAAAACTTTCCTT

o15.123

forward primer TDH3 from registry with HO binding tail

GCCCAGAATACCCTCCTTGACAGACAGTTTATTCCTGGCATCCACTAAATATAATG

o15.124

reverse primer TDH3 from registry with RE binding site

TATACTGCAGTTTGTTTGTTTATGTGTGTTTATTCGAAACTAAGTTC

o15.125

forward primer full gblock 1 with new promoter

GCCCAGAATACCCTCCTTGACAG

o15.126

reverse primer full gblock 1 with new promoter

CATGCTAACCCTTCCTCCAGGCT

o15.127

forward primer gblock 2 + tail

TCACAACAAGAATTTTGCAGAGGACCTAACCGAAGGAAAGTTTTCCTTCCCAACAATC

o15.128

reverse primer gblock 2 + tail

GGACTTGATAGAATTTTGTCTGGTTGACTCGGATGCAATGGAGGACCGAACAAT

o15.129

forward primer gblock 3

AACGTTATTGTTCGGTCCTCCATTGC

o15.130

reverse primer gblock 3 + tail

GTCCAGATTGACTCGAATGGGTGTAATGCCAGTATTTGACCAATGAATTGTAGCCTCAAG

o15.131

forward primer gblock 4

CTTGAGGCTACAATTCATTGGTCAAATAC

o15.132

reverse primer gblock 4 HO tail

TGTACGGGCGACAGTCACATCATGCCCCTGCAGGGTACCGCGAATTCCCC

o15.133

reverse primer gblock 4 HMG tail

CATGCGCCTATTATTACTTCAATAGATGGCAGGGTACCGCGAATTCCCC

o15.134

forward primer vector HO with HMG

GCTTTAATTTGCGGCCGGTACCCAATTCGCCAGGGGCATGATGTGACTGTCG

o15.135

forward primer vector HO without HMG (binds to gBlock4)

TCGCTATACTGGGGAATTCGCGGTACCCTGCAGGGGCATGATGTGACTGTCG

o15.136

reverse primer vector HO (binds to TDH3?)

TATATTTAGTGGATGCCAGGAATAAACTGTCTGTCAAGGAGGGTATTCTGGGC

o15.137

formard primer HMG+tail with RE

TATATCTAGACCCGCCGCCACCATGCAAAG

o15.138

reverse primer HMG+tail with restriction site

TATACTCGAGTCACTGTTGAGACCTCAAATCCTGTAAG

o15.139

forward primer promoter HMG terminator

TCGCTATACTGGGGAATTCGCGGTACCCTGCCATCTATTGAAGTAATAATAGGCGCATG

o15.140

reverse primer HMG + tail HO

TGTACGGGCGACAGTCACATCATGCCCCTGGCGAATTGGGTACCGGCCG

o15.141

reverse primer gblock 1 part 1 (binds to part2))

TCCTCAACGTCGTCCATCAGTAAGCTCGCTGTGTGCAACATTCCCACTACATTTTGAATG

o15.142

forward primer gblock 1 + tail that binds to HO pl

GCCCAGAATACCCTCCTTGACAGCGTCCAAGACATACTGCGTTTACG

o15.143

reverse primer HO + tail crappy ADH1

TCGACACGTAAACGCAGTATGTCTTGGACGCTGTCAAGGAGGGTATTCTGGGC

o15.144

forward primer gblock 1 + tail TDH3

GTTTCGAATAAACACACATAAACAAACAAATCTAGAACTAGTGGATCCATGGATTAC

o15.145

reverse primer TDH3 + tail for kozac

TCCATGGATCCACTAGTTCTAGATTTGTTTGTTTATGTGTGTTTATTCGAAACTAAGTTC