Difference between revisions of "Team:Paris Bettencourt/Protocols/titration-acid-phytic"

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       <li><b>Preparation of reagent solutions (not supllied) :</b></li>
 
       <li><b>Preparation of reagent solutions (not supllied) :</b></li>
 
           <ul>
 
           <ul>
−
           <li><i>Trichloroacetic acid  (50% w/v) : 100mL</i></li>
+
           <li><i><b>Trichloroacetic acid  (50% w/v) : 100mL</b></i></li>
 
           <li>Add 50g of thrichloroacetic to 60mL of distilled water and dissolve. Make to volume (100mL) with distilled water. (Stable for > 6 moths at 4°C)</li>
 
           <li>Add 50g of thrichloroacetic to 60mL of distilled water and dissolve. Make to volume (100mL) with distilled water. (Stable for > 6 moths at 4°C)</li>
−
           <li><i>Hydrochloric acid (0.66M) : 1L</i></li>
+
           <li><i><b>Hydrochloric acid (0.66M) : 1L</b></i></li>
 
           <li>Add 54.5mL of hydrochloric acid to 945.5mL of distilled wtaer an mix. (Store at room temperature)</li>
 
           <li>Add 54.5mL of hydrochloric acid to 945.5mL of distilled wtaer an mix. (Store at room temperature)</li>
−
           <li><i>Sodium hydroxide (0.75M) : 200mL</i></li>
+
           <li><i><b>Sodium hydroxide (0.75M) : 200mL</b></i></li>
 
           <li>Add 6g of sodium hydroxide pellets to 180mL of distilled water and dissolve. Make to volume (200mL) with distilled water. (Store at room temperature)</li>
 
           <li>Add 6g of sodium hydroxide pellets to 180mL of distilled water and dissolve. Make to volume (200mL) with distilled water. (Store at room temperature)</li>
−
           <li></li>
+
           <li><i><b>Phytic acid :</b></i></li>
−
           <li></li>
+
           <li>Pure phytic acid control sample may be required.</li>
−
          </ul>
+
          </ul>
−
       <li>
+
       <li><b>Sample extraction :</b></li>
−
    </ul>
+
          <ul>
 +
          <li>Weigh 1g of sample material.</li>
 +
          <li>Add 20mL of hydrochloric acid (0.66M).</li>
 +
          <li>Cover with foil and incubate for a minimum of 3 hours at room temperature.</li>
 +
          </u>
 +
      <li></li>
 +
      <li></li>
 +
      <li></li>
 +
</ul>
 
     </td>
 
     </td>
 
   </tr>
 
   </tr>
 
</table>
 
</table>
 
{{Paris_Bettencourt/footer}}
 
{{Paris_Bettencourt/footer}}

Revision as of 14:48, 28 August 2015

Titration of phytc acid using Megazyme© Kit
  • Preparation of reagent solutions (not supllied) :
    • Trichloroacetic acid (50% w/v) : 100mL
    • Add 50g of thrichloroacetic to 60mL of distilled water and dissolve. Make to volume (100mL) with distilled water. (Stable for > 6 moths at 4°C)
    • Hydrochloric acid (0.66M) : 1L
    • Add 54.5mL of hydrochloric acid to 945.5mL of distilled wtaer an mix. (Store at room temperature)
    • Sodium hydroxide (0.75M) : 200mL
    • Add 6g of sodium hydroxide pellets to 180mL of distilled water and dissolve. Make to volume (200mL) with distilled water. (Store at room temperature)
    • Phytic acid :
    • Pure phytic acid control sample may be required.
  • Sample extraction :
    • Weigh 1g of sample material.
    • Add 20mL of hydrochloric acid (0.66M).
    • Cover with foil and incubate for a minimum of 3 hours at room temperature.
      •          </u>

       </td>
 </tr>

    </table>