Difference between revisions of "Team:Paris Bettencourt/Protocols/titration-acid-phytic"
| Line 9: | Line 9: | ||
<li><b>Preparation of reagent solutions (not supllied) :</b></li> | <li><b>Preparation of reagent solutions (not supllied) :</b></li> | ||
<ul> | <ul> | ||
| − | <li><i><b>Trichloroacetic acid (50% w/v) : 100mL</b></i></li | + | <li><i><b>Trichloroacetic acid (50% w/v) : 100mL</b></i></li> |
Add 50g of thrichloroacetic to 60mL of distilled water and dissolve. Make to volume (100mL) with distilled water. (Stable for > 6 moths at 4°C)</li> | Add 50g of thrichloroacetic to 60mL of distilled water and dissolve. Make to volume (100mL) with distilled water. (Stable for > 6 moths at 4°C)</li> | ||
| − | <li><i><b>Hydrochloric acid (0.66M) : 1L</b></i></li | + | <li><i><b>Hydrochloric acid (0.66M) : 1L</b></i></li> |
Add 54.5mL of hydrochloric acid to 945.5mL of distilled wtaer an mix. (Store at room temperature)</li> | Add 54.5mL of hydrochloric acid to 945.5mL of distilled wtaer an mix. (Store at room temperature)</li> | ||
| − | <li><i><b>Sodium hydroxide (0.75M) : 200mL</b></i></li | + | <li><i><b>Sodium hydroxide (0.75M) : 200mL</b></i></li> |
Add 6g of sodium hydroxide pellets to 180mL of distilled water and dissolve. Make to volume (200mL) with distilled water. (Store at room temperature)</li> | Add 6g of sodium hydroxide pellets to 180mL of distilled water and dissolve. Make to volume (200mL) with distilled water. (Store at room temperature)</li> | ||
| − | <li><i><b>Phytic acid :</b></i></li | + | <li><i><b>Phytic acid :</b></i></li> |
Pure phytic acid control sample may be required.</li> | Pure phytic acid control sample may be required.</li> | ||
</ul> | </ul> | ||
Revision as of 14:50, 28 August 2015
Ferment It Yourself
iGEM Paris-Bettencourt 2O15
- Background
- Design
-
-
-
-
-
-
-
Vitamin A Vitamin B2 Vitamin B12 Phytase Riboswitch Differentiation on E. coli Differentiation on S. cerevisiae Manufacturing Idli and Micro-organisms Titration of phytc acid using Megazyme© Kit
- Preparation of reagent solutions (not supllied) :
- Trichloroacetic acid (50% w/v) : 100mL Add 50g of thrichloroacetic to 60mL of distilled water and dissolve. Make to volume (100mL) with distilled water. (Stable for > 6 moths at 4°C)
- Hydrochloric acid (0.66M) : 1L Add 54.5mL of hydrochloric acid to 945.5mL of distilled wtaer an mix. (Store at room temperature)
- Sodium hydroxide (0.75M) : 200mL Add 6g of sodium hydroxide pellets to 180mL of distilled water and dissolve. Make to volume (200mL) with distilled water. (Store at room temperature)
- Phytic acid : Pure phytic acid control sample may be required.
- Sample extraction :
- Weigh 1g of sample material.
- Add 20mL of hydrochloric acid (0.66M).
- Cover with foil and incubate for a minimum of 3 hours at room temperature.
</u>
</td> </tr>
</table>
