Difference between revisions of "Team:Paris Bettencourt/Protocols/Digestion"

 
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<table style="width:35%">
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{{Paris_Bettencourt/header}}
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{{Paris_Bettencourt/menu}}
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{{Paris_Bettencourt/notebookBanner}}
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<table>
 
   <tr>
 
   <tr>
 
     <td><b>Digestion Protocol</b><br/>
 
     <td><b>Digestion Protocol</b><br/>
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<li>Prepare the following mix:<br/>
 
<li>Prepare the following mix:<br/>
 
   <ul>
 
   <ul>
   <li>4μL of Enzyme 1
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   <li>4μL of Enzyme 1 Fast Digest</li>
   <li>4μL of Enzyme 2
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   <li>4μL of Enzyme 2 Fast Digest</li>
   <li>4μL of FastAP
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   <li>4μL of FastAP</li>
   <li>12μL of Fast Digest buffer 10X
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   <li>12μL of Fast Digest buffer 10X</li>
   <li>1 to 3 μg of DNA
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   <li>1 to 3 μg of DNA</li>
   <li>up to 120μL of water
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   <li>up to 120μL of water</li>
 
   </ul>
 
   </ul>
<li>mix by pipetting up and down
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<li>mix by pipetting up and down</li>
<li>incube 10min at 37°C
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<li>incube 10 to 20 minutes at 37°C</li>
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<li>incube 10 minutes at 68°C to inactivate the enzymes</li>
 
</ul>
 
</ul>
 
     </td>
 
     </td>

Latest revision as of 13:05, 3 September 2015


Digestion Protocol
  • Prepare the following mix:
    • 4μL of Enzyme 1 Fast Digest
    • 4μL of Enzyme 2 Fast Digest
    • 4μL of FastAP
    • 12μL of Fast Digest buffer 10X
    • 1 to 3 μg of DNA
    • up to 120μL of water
  • mix by pipetting up and down
  • incube 10 to 20 minutes at 37°C
  • incube 10 minutes at 68°C to inactivate the enzymes