Difference between revisions of "Team:Paris Bettencourt/Protocols/Digestion"
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<li>Prepare the following mix:<br/> | <li>Prepare the following mix:<br/> | ||
<ul> | <ul> | ||
| − | <li>4μL of Enzyme 1 | + | <li>4μL of Enzyme 1 Fast Digest</li> |
| − | <li>4μL of Enzyme 2 | + | <li>4μL of Enzyme 2 Fast Digest</li> |
| − | <li>4μL of FastAP | + | <li>4μL of FastAP</li> |
| − | <li>12μL of Fast Digest buffer 10X | + | <li>12μL of Fast Digest buffer 10X</li> |
| − | <li>1 to 3 μg of DNA | + | <li>1 to 3 μg of DNA</li> |
| − | <li>up to 120μL of water | + | <li>up to 120μL of water</li> |
</ul> | </ul> | ||
| − | <li>mix by pipetting up and down | + | <li>mix by pipetting up and down</li> |
| − | <li>incube | + | <li>incube 10 to 20 minutes at 37°C</li> |
| + | <li>incube 10 minutes at 68°C to inactivate the enzymes</li> | ||
</ul> | </ul> | ||
</td> | </td> | ||
Latest revision as of 13:05, 3 September 2015