Difference between revisions of "Team:Paris Bettencourt/Protocols/Ligation"
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| + | {{Paris_Bettencourt/header}} | ||
| + | {{Paris_Bettencourt/menu}} | ||
| + | {{Paris_Bettencourt/notebookBanner}} | ||
| − | <table | + | <table> |
<tr> | <tr> | ||
<td><b>Ligation Protocol</b><br> | <td><b>Ligation Protocol</b><br> | ||
| Line 9: | Line 12: | ||
<li>vector 100ng | <li>vector 100ng | ||
<li>insert 300ng | <li>insert 300ng | ||
| − | <li>T4 DNA ligase buffer 10X | + | <li>2µL T4 DNA ligase buffer 10X |
| − | <li>T4 DNA ligase | + | <li>1µL T4 DNA ligase |
<li>up to 20µL of water | <li>up to 20µL of water | ||
</ul> | </ul> | ||
| − | <li>incubate | + | <li>incubate 10 to 15 minutes at 22°C |
| + | <li>incubate 5 minutes at 70°C | ||
| + | |||
</ul> | </ul> | ||
</td> | </td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
Latest revision as of 18:36, 7 September 2015