Difference between revisions of "Team:Paris Bettencourt/Notebook/Manufacturing"
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<li>Rice flour: 1,9%</li> | <li>Rice flour: 1,9%</li> | ||
</ul> | </ul> | ||
− | We can see that the more ingredients we put, the more the yeasts | + | We can see that the more ingredients we put, the more the yeasts survived. |
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<h1 class="date two">August 11th</h1> | <h1 class="date two">August 11th</h1> | ||
+ | <h2>Goal</h2> | ||
+ | While waiting for the cubes results, we are thinking about the next step: finding a media to grow the strains, easy to give to the population and to use afterward. The perfect media would be an edible one, that we could incorporate to the cube, instead of the water.<br> | ||
<h2>Procedure</h2> | <h2>Procedure</h2> | ||
+ | <ul> | ||
+ | <li>Heat 3 potatoes in 750ml of water</li> | ||
+ | <li>Let the water boil (sterilisation)</li> | ||
+ | <li>Save the water and stock it in a bottle </li> | ||
+ | <li>Put 10ml of this potatoe water in a culture tube</li> | ||
+ | <li>Add 20μL of geneticin (100mg/ml)</li> | ||
+ | <li>Inoculate with Sc. M Cherry</li> | ||
+ | <li>Put in the incubator at 30°C for 2 days</li> | ||
+ | <li>Repeat the process with G1513, replacing the geneticin by 5μL of erythromycin (20mg/ml)</li> | ||
+ | </ul> | ||
<br><br> | <br><br> | ||
<h1 class="date two">August 12th</h1> | <h1 class="date two">August 12th</h1> | ||
+ | <h2>Results of the cube tests</h2> | ||
+ | The yeasts survived well: 42%. But strangely, the number of cells which are supposed to be Lactococcus, in the plates of M17 glucose with erythromycin, is the same as in the plates with YPD and geneticin, selecting the yeasts.<br> | ||
+ | Therefore, we checked the production of RFP, since Sc. M Cherry produces RFP but not G1513.<br> | ||
+ | Unfortunately, we came to the conclusion that the cells in the M17 plates were yeasts. Actually, Sc. M Cherry resist to erythromycin and is therefore growing in the plate.<br> | ||
+ | <h2>Goal</h2> | ||
+ | We need a chemical that will allow us to select the Lactococcus and kill the yeasts.<br> | ||
+ | After some researchs, we found out that cycloheximide could be helpful and ordered it. While waiting for this chemical, we cn't work with G1513. | ||
<br><br> | <br><br> | ||
<h1 class="date two">August 13th</h1> | <h1 class="date two">August 13th</h1> | ||
+ | |||
<br><br> | <br><br> |
Revision as of 14:55, 8 September 2015