Difference between revisions of "Team:Paris Bettencourt/Notebook/Riboswitch"
Line 462: | Line 462: | ||
Waited 40 min on the bench (25°C) | Waited 40 min on the bench (25°C) | ||
− | <h2>Goal | + | <h2>Goal 3 </h2> |
Transformation of L* | Transformation of L* | ||
Line 495: | Line 495: | ||
<td class="tg-031e">-</td> | <td class="tg-031e">-</td> | ||
<td class="tg-031e">3 uL</td> | <td class="tg-031e">3 uL</td> | ||
− | <td class="tg-031e"></td> | + | <td class="tg-031e">-</td> |
</tr> | </tr> | ||
</table> | </table> | ||
Line 501: | Line 501: | ||
90 sec heat shock<br> | 90 sec heat shock<br> | ||
On saturday they were growing but no fluorescence | On saturday they were growing but no fluorescence | ||
+ | |||
+ | <br><br> | ||
+ | <h1 class="date two">August 10th</h1> | ||
+ | |||
+ | <h2>Goal 1 </h2> | ||
+ | Colony PCR my T* | ||
+ | |||
+ | <h2>Procedure</h2> | ||
+ | Pick up 8 colonies on my two T* plates <br> | ||
+ | Put some bacteria in PCR tube + 2 uL of 148 and 149 (iGEM verification forward and backward) ; <br> | ||
+ | 10uM + 50 uL of Master Mix Phusion (last in the tubes) + 45 uL of water (first in the tube) <br> | ||
+ | Waiting for a 1577 bp fragment on a gel |
Revision as of 09:34, 9 September 2015