Difference between revisions of "Team:Paris Bettencourt/Notebook/Riboswitch"
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<br> | <br> | ||
It is quiet bad | It is quiet bad | ||
+ | |||
+ | <br><br> | ||
+ | <h1 class="date two">August 11th</h1> | ||
+ | |||
+ | <h2>Goal 1 </h2> | ||
+ | Do a new PCR of FINAL (G-block) and of pSB1C3 associated with RFP | ||
+ | |||
+ | <h2>Procedure</h2> | ||
+ | |||
+ | <style type="text/css"> | ||
+ | .tg {border-collapse:collapse;border-spacing:0;} | ||
+ | .tg td{font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} | ||
+ | .tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} | ||
+ | </style> | ||
+ | <table class="tg"> | ||
+ | <tr> | ||
+ | <th class="tg-031e"></th> | ||
+ | <th class="tg-031e">Template</th> | ||
+ | <th class="tg-031e">Primers</th> | ||
+ | <th class="tg-031e">Master Mix Phusion</th> | ||
+ | <th class="tg-031e">Water</th> | ||
+ | <th class="tg-031e">Time of elongation (72°C)</th> | ||
+ | <th class="tg-031e">T°C annealing</th> | ||
+ | <th class="tg-031e">Lenght</th> | ||
+ | <th class="tg-031e">Primers</th> | ||
+ | <th class="tg-031e">Nanodrop after purify (ng/uL)</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">LP1*</td> | ||
+ | <td class="tg-031e">5ng</td> | ||
+ | <td class="tg-031e">2uL</td> | ||
+ | <td class="tg-031e">25uL</td> | ||
+ | <td class="tg-031e">QSP for 50uL</td> | ||
+ | <td class="tg-031e">2 min</td> | ||
+ | <td class="tg-031e">56°C</td> | ||
+ | <td class="tg-031e">2078</td> | ||
+ | <td class="tg-031e">152 - 153</td> | ||
+ | <td class="tg-031e">68.3</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">LP2*</td> | ||
+ | <td class="tg-031e">10ng</td> | ||
+ | <td class="tg-031e">2uL</td> | ||
+ | <td class="tg-031e">25uL</td> | ||
+ | <td class="tg-031e">QSP for 50uL</td> | ||
+ | <td class="tg-031e">2 min</td> | ||
+ | <td class="tg-031e">56°C</td> | ||
+ | <td class="tg-031e">2078</td> | ||
+ | <td class="tg-031e">152 - 153</td> | ||
+ | <td class="tg-031e">133.5</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">LP3*</td> | ||
+ | <td class="tg-031e">2ng</td> | ||
+ | <td class="tg-031e">2uL</td> | ||
+ | <td class="tg-031e">25uL</td> | ||
+ | <td class="tg-031e">QSP for 50uL</td> | ||
+ | <td class="tg-031e">2 min</td> | ||
+ | <td class="tg-031e">56°C</td> | ||
+ | <td class="tg-031e">2078</td> | ||
+ | <td class="tg-031e">152 - 153</td> | ||
+ | <td class="tg-031e">127.8</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">F1*</td> | ||
+ | <td class="tg-031e">2ng</td> | ||
+ | <td class="tg-031e">2uL</td> | ||
+ | <td class="tg-031e">25uL</td> | ||
+ | <td class="tg-031e">QSP for 50uL</td> | ||
+ | <td class="tg-031e">1 min</td> | ||
+ | <td class="tg-031e">58°C</td> | ||
+ | <td class="tg-031e">1314</td> | ||
+ | <td class="tg-031e">150 - 151</td> | ||
+ | <td class="tg-031e">98</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">F2*</td> | ||
+ | <td class="tg-031e">3ng</td> | ||
+ | <td class="tg-031e">2uL</td> | ||
+ | <td class="tg-031e">25uL</td> | ||
+ | <td class="tg-031e">QSP for 50uL</td> | ||
+ | <td class="tg-031e">1 min</td> | ||
+ | <td class="tg-031e">58°C</td> | ||
+ | <td class="tg-031e">1314</td> | ||
+ | <td class="tg-031e">150 - 151</td> | ||
+ | <td class="tg-031e">102</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">F3*</td> | ||
+ | <td class="tg-031e">1ng</td> | ||
+ | <td class="tg-031e">2uL</td> | ||
+ | <td class="tg-031e">25uL</td> | ||
+ | <td class="tg-031e">QSP for 50uL</td> | ||
+ | <td class="tg-031e">1 min</td> | ||
+ | <td class="tg-031e">58°C</td> | ||
+ | <td class="tg-031e">1314</td> | ||
+ | <td class="tg-031e">150 - 151</td> | ||
+ | <td class="tg-031e">58</td> | ||
+ | </tr> |
Revision as of 09:45, 10 September 2015