Difference between revisions of "Team:Exeter/Protocols"
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/2/26/Exeter_MgCl2_CaCl2_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/2/2e/Exeter_CaCl2_MgCl2.png" style="height:250px"/></a> | <a target="_blank" href="https://static.igem.org/mediawiki/2015/2/26/Exeter_MgCl2_CaCl2_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/2/2e/Exeter_CaCl2_MgCl2.png" style="height:250px"/></a> | ||
<a target="_blank" href="https://static.igem.org/mediawiki/2015/a/ab/Exeter_Glycerol_recipe.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/f/f8/Exeter_glycerol_recipe.png" style="height:250px"/></a> | <a target="_blank" href="https://static.igem.org/mediawiki/2015/a/ab/Exeter_Glycerol_recipe.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/f/f8/Exeter_glycerol_recipe.png" style="height:250px"/></a> | ||
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/9/99/Exeter_Qubit_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/3/3f/Exeter_qubit.png" style="height:250px"/></a> | <a target="_blank" href="https://static.igem.org/mediawiki/2015/9/99/Exeter_Qubit_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/3/3f/Exeter_qubit.png" style="height:250px"/></a> | ||
<a target="_blank" href="https://static.igem.org/mediawiki/2015/3/33/Exeter_DNA_rehydration_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/c/ca/Exeter_DNA_rehydration.png" style="height:250px"/></a> | <a target="_blank" href="https://static.igem.org/mediawiki/2015/3/33/Exeter_DNA_rehydration_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/c/ca/Exeter_DNA_rehydration.png" style="height:250px"/></a> | ||
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<ul> | <ul> | ||
<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/7/77/Exeter_NEB_transformation_SOP.pdf"><em>E.coli</em> transformation.</a></li> | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/7/77/Exeter_NEB_transformation_SOP.pdf"><em>E.coli</em> transformation.</a></li> | ||
| − | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/e/e6/Exeter_gBlock_HiFi_SOP.pdf"></a></li> | + | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/e/e6/Exeter_gBlock_HiFi_SOP.pdf">gBlock Prep. and HiFi assembly.</a></li> |
| − | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/8/83/Exeter_competent_cells_SOP.pdf"></a></li> | + | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/8/83/Exeter_competent_cells_SOP.pdf">Competent cells preparation.</a></li> |
| − | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/c/c7/Exeter_protein_extraction_BB_SOP.pdf"></a></li> | + | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/c/c7/Exeter_protein_extraction_BB_SOP.pdf">Protein extraction (BugBuster).</a></li> |
| − | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/1/1f/Exeter_LB_recipe.pdf"></a></li> | + | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/1/1f/Exeter_LB_recipe.pdf">LB agar broth recipe.</a></li> |
| − | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/d/d6/Exeter_gel_electrophoresis_SOP.pdf"></a></li> | + | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/d/d6/Exeter_gel_electrophoresis_SOP.pdf">Agarose gel recipe.</a></li> |
| − | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/d/d5/Exeter_running_gel_electrophoresis.pdf"></a></li> | + | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/d/d5/Exeter_running_gel_electrophoresis.pdf">Running gel electrophoresis.</a></li> |
| − | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/7/78/Exeter_autoclave_SOP.pdf"></a></li> | + | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/7/78/Exeter_autoclave_SOP.pdf">Using the autoclave.</a></li> |
| − | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/0/06/Exeter_DNA_restriction_digest.pdf"></a></li> | + | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/0/06/Exeter_DNA_restriction_digest.pdf">DNA restriction digest.</a></li> |
| − | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/d/d0/Exeter_Fastdigest_SOP.pdf"></a></li> | + | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/d/d0/Exeter_Fastdigest_SOP.pdf">Thermo scientific FastDigest.</a></li> |
| − | <li><a target="_blank" href="https://2015.igem.org/File:Exeter_gel_extraction_SOP.pdf"></a></li> | + | <li><a target="_blank" href="https://2015.igem.org/File:Exeter_gel_extraction_SOP.pdf">DNA gel extraction.</a></li> |
| − | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/6/62/Exeter_glycerol_stocks_SOP.pdf"></a></li> | + | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/6/62/Exeter_glycerol_stocks_SOP.pdf">Cell glycerol stocks.</a></li> |
| − | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/7/7a/Exeter_Promega_ligation_SOP.pdf"></a></li> | + | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/7/7a/Exeter_Promega_ligation_SOP.pdf">Promega DNA ligation.</a></li> |
| − | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/f/f8/Exeter_miniprep_SOP.pdf"></a></li> | + | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/f/f8/Exeter_miniprep_SOP.pdf">Qiagen DNA miniprep.</a></li> |
| − | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/c/c2/Exeter_liquid_cultures_SOP.pdf"></a></li> | + | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/c/c2/Exeter_liquid_cultures_SOP.pdf">Liquid cell cultures.</a></li> |
| − | <li><a target="_blank" href="">< | + | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/2/26/Exeter_MgCl2_CaCl2_SOP.pdf">MgCl<sub>2</sub> and CaCl<sub>2</sub> recipes.</a></li> |
| − | < | + | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/a/ab/Exeter_Glycerol_recipe.pdf">Sterile 40% glycerol recipe.</a></li> |
| − | <li><a target="_blank" href=""></a></li> | + | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/9/99/Exeter_Qubit_SOP.pdf">Qubit for DNA concentration.</a></li> |
| − | <li><a target="_blank" href=""></a></li> | + | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/3/33/Exeter_DNA_rehydration_SOP.pdf">Dried DNA rehydration.</a></li> |
| − | <li><a target="_blank" href=""></a></li> | + | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/7/71/Exeter_S30_cell_free_kit_SOP.pdf">Promega S30 cell free kit.</a></li> |
| − | <li><a target="_blank" href=""></a></li> | + | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/3/3d/Exeter_solid_agar_recipe.pdf">Solid agar recipe for agar plates.</a></li> |
| − | <li><a target="_blank" href=""></a></li> | + | |
</ul> | </ul> | ||
Revision as of 22:23, 12 September 2015
Standard Operating Procedures (SOPs)
Use the mouse or touch screen to scroll through our SOPs. Click on an image to bring up the corresponding protocol.
- E.coli transformation.
- gBlock Prep. and HiFi assembly.
- Competent cells preparation.
- Protein extraction (BugBuster).
- LB agar broth recipe.
- Agarose gel recipe.
- Running gel electrophoresis.
- Using the autoclave.
- DNA restriction digest.
- Thermo scientific FastDigest.
- DNA gel extraction.
- Cell glycerol stocks.
- Promega DNA ligation.
- Qiagen DNA miniprep.
- Liquid cell cultures.
- MgCl2 and CaCl2 recipes.
- Sterile 40% glycerol recipe.
- Qubit for DNA concentration.
- Dried DNA rehydration.
- Promega S30 cell free kit.
- Solid agar recipe for agar plates.