Difference between revisions of "Team:Exeter/Protocols"
| Line 141: | Line 141: | ||
<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/3/3d/Exeter_solid_agar_recipe.pdf">Solid agar recipe for agar plates.</a></li> | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/3/3d/Exeter_solid_agar_recipe.pdf">Solid agar recipe for agar plates.</a></li> | ||
</ul> | </ul> | ||
| + | |||
| + | |||
| + | <div class="containerSlider400" style="float:right"> | ||
| + | <div class="slider400"> | ||
| + | |||
| + | <div class="slide"><a href="https://static.igem.org/mediawiki/2015/9/97/Exeter_protocols.zip"><img src="https://static.igem.org/mediawiki/2015/7/75/Exeter_protocols_download.png" title="Download all protocols."></a></div> | ||
</div> | </div> | ||
Revision as of 23:11, 12 September 2015
Standard Operating Procedures (SOPs)
Use the mouse or touch screen to scroll through our SOPs. Click on an image to bring up the corresponding protocol.
- E.coli transformation.
- gBlock Prep. and HiFi assembly.
- Competent cells preparation.
- Protein extraction (BugBuster).
- LB agar broth recipe.
- Agarose gel recipe.
- Running gel electrophoresis.
- Using the autoclave.
- DNA restriction digest.
- Thermo scientific FastDigest.
- DNA gel extraction.
- Cell glycerol stocks.
- Promega DNA ligation.
- Qiagen DNA miniprep.
- Liquid cell cultures.
- MgCl2 and CaCl2 recipes.
- Sterile 40% glycerol recipe.
- Qubit for DNA concentration.
- Dried DNA rehydration.
- Promega S30 cell free kit.
- Solid agar recipe for agar plates.
