Difference between revisions of "Team:Exeter/Protocols"

 
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<div class="container">
  
 
<div id="container">
 
<div id="container">
  
  
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<h1>Standard Operating Procedures (SOPs)</h1>
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<h1>Protocols</h1>
  
  
 
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<h5>
 
<h5>
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<center>Use the mouse or touch screen to scroll through our SOPs. Click on an image to bring up the corresponding protocol.</center>
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<center>Use the arrow keys or touch screen to scroll through our SOPs. Click on an image to bring up the corresponding protocol.</center>
 
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/7/77/Exeter_NEB_transformation_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/b/b5/Exeter_transformation.png" style="height:450px"/></a>
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/7/77/Exeter_NEB_transformation_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/3/39/Exeter_NEB_transformation.png" style="height:250px"/></a>
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<img class="cover" src="https://static.igem.org/mediawiki/2015/2/29/GBlock_SOP.png" style="height:450px"/>
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/e/e6/Exeter_gBlock_HiFi_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/2/29/GBlock_SOP.png" style="height:250px"/></a>
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<img class="cover" src="https://static.igem.org/mediawiki/2015/8/80/Exeter_comp_cells_SOP.png" style="height:450px"/>
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/8/83/Exeter_competent_cells_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/8/80/Exeter_comp_cells_SOP.png" style="height:250px"/></a>
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<img class="cover" src="https://static.igem.org/mediawiki/2015/b/b5/Exeter_transformation.png" style="height:450px"/>
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/c/c7/Exeter_protein_extraction_BB_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/c/ca/Exeter_protein_extraction_BB.png" style="height:250px"/></a>
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<img class="cover" src="https://static.igem.org/mediawiki/2015/b/b5/Exeter_transformation.png" style="height:450px"/>
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/1/1f/Exeter_LB_recipe.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/2/24/Exeter_LB_broth.png" style="height:250px"/></a>
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<img class="cover" src="https://static.igem.org/mediawiki/2015/b/b5/Exeter_transformation.png" style="height:450px"/>
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/d/d6/Exeter_gel_electrophoresis_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/2/25/Exeter_agarose_gel_recipe.png" style="height:250px"/></a>
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/d/d5/Exeter_running_gel_electrophoresis.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/0/05/Exeter_running_gel_electrophoresis.png" style="height:250px"/></a>
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/7/78/Exeter_autoclave_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/a/a5/Exeter_autoclave.png" style="height:250px"/></a>
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/0/06/Exeter_DNA_restriction_digest.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/5/5f/Exeter_restriction_digest.png" style="height:250px"/></a>
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/d/d0/Exeter_Fastdigest_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/a/a8/Exeter_fastdigest.png" style="height:250px"/></a>
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<a target="_blank" href="https://2015.igem.org/File:Exeter_gel_extraction_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/5/5e/Exeter_gel_extraction.png" style="height:250px"/></a>
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/6/62/Exeter_glycerol_stocks_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/3/32/Exeter_glycerol_stocks.png" style="height:250px"/></a>
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/7/7a/Exeter_Promega_ligation_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/6/6f/Exeter_DNA_ligation.png" style="height:250px"/></a>
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/f/f8/Exeter_miniprep_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/6/61/Exeter_miniprep.png" style="height:250px"/></a>
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/c/c2/Exeter_liquid_cultures_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/5/5d/Exeter_overnight_cultures.png" style="height:250px"/></a>
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/2/26/Exeter_MgCl2_CaCl2_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/2/2e/Exeter_CaCl2_MgCl2.png" style="height:250px"/></a>
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/a/ab/Exeter_Glycerol_recipe.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/f/f8/Exeter_glycerol_recipe.png" style="height:250px"/></a>
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/9/99/Exeter_Qubit_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/3/3f/Exeter_qubit.png" style="height:250px"/></a>
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/3/33/Exeter_DNA_rehydration_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/c/ca/Exeter_DNA_rehydration.png" style="height:250px"/></a>
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/7/71/Exeter_S30_cell_free_kit_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/b/b6/Exeter_S30_cell_free.png" style="height:250px"/></a>
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/3/3d/Exeter_solid_agar_recipe.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/9/94/Exeter_solid_agar.png" style="height:250px"/></a>
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/e/ea/Exeter_SDS_PAGE_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/0/0e/Exeter_sds_page_sop.png" style="height:250px"/></a>
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<a target="_blank" href="https://static.igem.org/mediawiki/2015/f/f6/Exeter_Q5_mutagenesis_sop.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/9/95/Exeter_Q5_mutagenesis_primers.png" style="height:250px"/></a>
 
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<div class="sop">
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<div class="containerSlider400" style="float:right">
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<div class="sliderstatic400">
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<div class="slide"><a href="https://static.igem.org/mediawiki/2015/9/97/Exeter_protocols.zip"><img src="https://static.igem.org/mediawiki/2015/7/75/Exeter_protocols_download.png" title="Click the image to download all protocols."></a></div>
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</div>
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</div>
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<ul>
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<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/7/77/Exeter_NEB_transformation_SOP.pdf"><em>E.coli</em> transformation.</a></li>
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<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/e/e6/Exeter_gBlock_HiFi_SOP.pdf">gBlock Prep. and HiFi assembly.</a></li>
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<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/8/83/Exeter_competent_cells_SOP.pdf">Competent cells preparation.</a></li>
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<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/c/c7/Exeter_protein_extraction_BB_SOP.pdf">Protein extraction (BugBuster).</a></li>
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<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/1/1f/Exeter_LB_recipe.pdf">LB agar broth recipe.</a></li>
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<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/d/d6/Exeter_gel_electrophoresis_SOP.pdf">Agarose gel recipe.</a></li>
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<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/d/d5/Exeter_running_gel_electrophoresis.pdf">Running gel electrophoresis.</a></li>
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<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/7/78/Exeter_autoclave_SOP.pdf">Using the autoclave.</a></li>
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<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/0/06/Exeter_DNA_restriction_digest.pdf">DNA restriction digest.</a></li>
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<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/d/d0/Exeter_Fastdigest_SOP.pdf">Thermo scientific FastDigest.</a></li>
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<li><a target="_blank" href="https://2015.igem.org/File:Exeter_gel_extraction_SOP.pdf">DNA gel extraction.</a></li>
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<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/6/62/Exeter_glycerol_stocks_SOP.pdf">Cell glycerol stocks.</a></li>
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<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/7/7a/Exeter_Promega_ligation_SOP.pdf">Promega DNA ligation.</a></li>
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<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/f/f8/Exeter_miniprep_SOP.pdf">Qiagen DNA miniprep.</a></li>
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<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/c/c2/Exeter_liquid_cultures_SOP.pdf">Liquid cell cultures.</a></li>
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<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/2/26/Exeter_MgCl2_CaCl2_SOP.pdf">MgCl<sub>2</sub> and CaCl<sub>2</sub> recipes.</a></li>
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<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/a/ab/Exeter_Glycerol_recipe.pdf">Sterile 40% glycerol recipe.</a></li>
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<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/9/99/Exeter_Qubit_SOP.pdf">Qubit for DNA concentration.</a></li>
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<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/3/33/Exeter_DNA_rehydration_SOP.pdf">Dried DNA rehydration.</a></li>
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<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/7/71/Exeter_S30_cell_free_kit_SOP.pdf">Promega S30 cell free kit.</a></li>
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<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/3/3d/Exeter_solid_agar_recipe.pdf">Solid agar recipe for agar plates.</a></li>
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</ul>
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   <ul class="pager">
 
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     <li class="previous"><a href="https://2015.igem.org/Team:Exeter/Design"><span aria-hidden="true">&larr;</span>Design</a></li>
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     <li class="previous"><a href="https://2015.igem.org/Team:Exeter/Results"><span aria-hidden="true">&larr;</span>Results</a></li>
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     <li class="next"><a href="https://2015.igem.org/Team:Exeter/diary">Diary<span aria-hidden="true">&rarr;</span></a></li>
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     <li class="next"><a href="https://2015.igem.org/Team:Exeter/Safety">Safety<span aria-hidden="true">&rarr;</span></a></li>
 
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Latest revision as of 14:31, 14 September 2015

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