Difference between revisions of "Team:Paris Bettencourt/Notebook/Riboswitch"
Line 1,355: | Line 1,355: | ||
<h2>Result</h2> | <h2>Result</h2> | ||
<img src="https://static.igem.org/mediawiki/2015/4/4f/090909.png" width="350px"> | <img src="https://static.igem.org/mediawiki/2015/4/4f/090909.png" width="350px"> | ||
+ | Then m iniprep of 22, 23 (+24?), 24 <br> | ||
+ | Also plated LB + Cm<br> | ||
+ | And liquid culture | ||
+ | |||
+ | <br><br> | ||
+ | <h1 class="date two">August 21th</h1> | ||
+ | |||
+ | <h2>Goal</h2> | ||
+ | Digestion of my mini prep from yesterday | ||
+ | <h2>Procedure</h2> | ||
+ | Nanodrop : <br> | ||
+ | 22 = 23.5ng/uL<br> | ||
+ | 23 = 53.7ng/uL<br> | ||
+ | 24 = 25.5ng/uL<br> | ||
+ | |||
+ | <style type="text/css"> | ||
+ | .tg {border-collapse:collapse;border-spacing:0;} | ||
+ | .tg td{font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} | ||
+ | .tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} | ||
+ | </style> | ||
+ | <table class="tg"> | ||
+ | <tr> | ||
+ | <th class="tg-031e"></th> | ||
+ | <th class="tg-031e">22</th> | ||
+ | <th class="tg-031e">23</th> | ||
+ | <th class="tg-031e">24</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">DNA</td> | ||
+ | <td class="tg-031e">16</td> | ||
+ | <td class="tg-031e">16</td> | ||
+ | <td class="tg-031e">16</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">Buffer</td> | ||
+ | <td class="tg-031e">2</td> | ||
+ | <td class="tg-031e">2</td> | ||
+ | <td class="tg-031e">2</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">Hind III</td> | ||
+ | <td class="tg-031e">1</td> | ||
+ | <td class="tg-031e">1</td> | ||
+ | <td class="tg-031e">1</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">Nco I</td> | ||
+ | <td class="tg-031e">1</td> | ||
+ | <td class="tg-031e">1</td> | ||
+ | <td class="tg-031e">1</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">TOTAL</td> | ||
+ | <td class="tg-031e">20</td> | ||
+ | <td class="tg-031e">20</td> | ||
+ | <td class="tg-031e">20</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">Expected length</td> | ||
+ | <td class="tg-031e">2040 bp (no insertion, recircularisation)</td> | ||
+ | <td class="tg-031e">1315 and 2026 bp (insertion)</td> | ||
+ | <td class="tg-031e">2040 bp</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
+ | 37°C 20 min<br> | ||
+ | Gel order : GR1kb , 22, 23, 24, GR1kb<br> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/b/b9/9876.png" width="350px"> | ||
+ | Exactly what I was expecting<br> | ||
+ | Band 23 : 3700 = plasmid undigested ?? | ||
+ | <br> | ||
+ | <br> | ||
+ | New gel <br> | ||
+ | Order : GR 1kb, 23, 23 d, 24, 24d | ||
+ | <img src="https://static.igem.org/mediawiki/2015/9/98/9090.png" width="350px"> |
Revision as of 11:48, 16 September 2015