Difference between revisions of "Team:Exeter/Parts"

Line 130: Line 130:
 
<h4 id="K1586000"><a href="http://parts.igem.org/Part:BBa_K1586000">BBa_K1586000: Synthetic toehold riboswitch J23100 (GreenFET1J)</a></h4>
 
<h4 id="K1586000"><a href="http://parts.igem.org/Part:BBa_K1586000">BBa_K1586000: Synthetic toehold riboswitch J23100 (GreenFET1J)</a></h4>
  
<div class="containerSlider500" style="float:right">
+
<div class="containerSlider400" style="float:right">
<div class="sliderstatic500">
+
<div class="sliderstatic400">
  
 
<div class="slide"><a href="http://parts.igem.org/Part:BBa_K1586000"><img src="https://static.igem.org/mediawiki/2015/6/64/Exeter_K1586000_features.png" title="Figure 1: Features of K1586000."></a></div>
 
<div class="slide"><a href="http://parts.igem.org/Part:BBa_K1586000"><img src="https://static.igem.org/mediawiki/2015/6/64/Exeter_K1586000_features.png" title="Figure 1: Features of K1586000."></a></div>

Revision as of 16:57, 18 September 2015

Parts

Toeholds

For our project, four biobricks were submitted to the registry, all of which are added as basic parts. Although two of the parts contain multiple parts from the iGEM registry, these have not been entered as composite parts. The main reason for this is that the inclusion of biobrick assembly scars in certain parts of the toehold structure could mess with the structure and function of the toehold, and would impose additional constraints upon the toehold design. Given this, we believe that it would be most useful to the synthetic biology community if this was characterised was a basic part which can be swapped between plasmid vectors as required, and the target changed using Q5 site-directed mutagenesis.

BBa_K1586000: Synthetic toehold riboswitch J23100 (GreenFET1J)

The first part submitted is a synthetic toehold switch which has been placed under the control of a standard constitutive promoter (J23100) and modified into a biobrick, namely through the removal of a PstI site at position 844. Figure 1 shows annotated features for K1586000.

Reporters

  • Contact us:
    exeterigem@gmail.com