Difference between revisions of "Team:Hamburg/Parts"

 
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<h2> our parts (in progress):</p>
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<h1>Parts</h1>
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<ul>
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<li><a href="http://parts.igem.org/Part:BBa_K1860700"> miRNA2911</a></li>
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</ul>
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<h2> Part Documentation</h2>
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<p>Our ultimate goal was to create a Biobrick enabling light-inducible miRNA2911 production and heat-inducible cell lysis for the release afterwards in one plasmid (see figure). Despite not reaching this goal so far, we completed important intermediate steps successfully heading towards it.</p>
  
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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    <img src="https://static.igem.org/mediawiki/2015/b/b6/Hamburg_parts4.jpg"/>
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    <figcaption></figcaption>
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<p>Two <a href="https://2015.igem.org/Team:Hamburg/Basic_Part">basic parts</a> contain the <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1860700">miRNA</a> and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1860701">GroEL</a>. Two <a href="https://2015.igem.org/Team:Hamburg/Composite_Part">composite parts</a> contain a <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1860702">constitutive promoter + miRNA</a> system (where the constitutive promoter replaces the light-inducible promoter as an intermediate step) and a <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1860703">GroEL + blue pigment</a> system (where the blue pigment replaces the cell lysis protein PezT for now). Further steps would be to design a <i>light promoter + miRNA</i> biobrick and a <i>GroEL + PezT</i> Biobrick, to then combine them by 3A-Assembly for our ultimate goal.</p>
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<h4>Note</h4>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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    <img src="https://static.igem.org/mediawiki/2015/8/89/Hamburg_mainpic.jpg" alt="Our concept" />
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    <figcaption>This figure delineates the basic steps of our ultimate goal.</figcaption>
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<h4>Adding parts to the registry</h4>
 
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
 
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
 
  
  
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<h4>What information do I need to start putting my parts on the Registry?</h4>
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<h3>Team Parts</h3>
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<p>The information needed to initially create a part on the Registry is:</p>
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<ul>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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</ul>
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<p>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h4>Inspiration</h4>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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</ul>
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<h4>Part Table </h4>
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<groupparts>iGEM015 Example</groupparts>
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<groupparts>iGEM015 Hamburg</groupparts>
 
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Latest revision as of 23:27, 18 September 2015

Parts

Our ultimate goal was to create a Biobrick enabling light-inducible miRNA2911 production and heat-inducible cell lysis for the release afterwards in one plasmid (see figure). Despite not reaching this goal so far, we completed important intermediate steps successfully heading towards it.

Two basic parts contain the miRNA and GroEL. Two composite parts contain a constitutive promoter + miRNA system (where the constitutive promoter replaces the light-inducible promoter as an intermediate step) and a GroEL + blue pigment system (where the blue pigment replaces the cell lysis protein PezT for now). Further steps would be to design a light promoter + miRNA biobrick and a GroEL + PezT Biobrick, to then combine them by 3A-Assembly for our ultimate goal.

Our concept
This figure delineates the basic steps of our ultimate goal.

Team Parts

<groupparts>iGEM015 Hamburg</groupparts>


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