Difference between revisions of "Team:Aachen/Basic Part"
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− | The | + | The characterization of the coding sequence was done with a RBS ([http://parts.igem.org/Part:BBa_B0034 B0034]) in a T7 driven expression backbone. The expression of this coding sequence is guaranteed when [http://parts.igem.org/Part:BBa_K1585210 B0034.Mdh] is used. |
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By far the highest formaldehyde production was observed in the BL21 Gold (DE3) cells 6 h after induction cultivated at 37 °C despite its formation of inclusion bodies. | By far the highest formaldehyde production was observed in the BL21 Gold (DE3) cells 6 h after induction cultivated at 37 °C despite its formation of inclusion bodies. | ||
− | To show the significance of the production of formaldehyde the assay was conducted with the Mdh expressing BL21 Gold (DE3) cultivated in the [[Team:Aachen/Lab/Methanol/Labeling_Experiment|labled methanol experiment]] along with the BL21 Gold (DE3) expressing GlgC as a negative control, both in multiple replicates. | + | To show the significance of the production of formaldehyde the assay was conducted with the Mdh expressing BL21 Gold (DE3) cultivated in the [[Team:Aachen/Lab/Methanol/Characterization#Labeling_Experiment|labled methanol experiment]] along with the BL21 Gold (DE3) expressing GlgC as a negative control, both in multiple replicates. |
Latest revision as of 02:24, 19 September 2015