Difference between revisions of "Team:Paris Bettencourt/Practices/DILambda"
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Accuracy comparable to existing devices or industry standards. | Accuracy comparable to existing devices or industry standards. | ||
Design for cheap assembly and mass production | Design for cheap assembly and mass production | ||
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+ | 1/ One mass production success is the desktop spectrometry kit 3.0 from PublicLab (Fig. 1). (It is actually a spectrometer, not a spectrophotometer!) It looks simple and elegant, produces quality results, and has attracted a community of users. | ||
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<img src="https://static.igem.org/mediawiki/2015/4/47/ParisBettencourt_juanma04.jpg" style="width: 100%"> | <img src="https://static.igem.org/mediawiki/2015/4/47/ParisBettencourt_juanma04.jpg" style="width: 100%"> | ||
− | <p class="caption"><b>Figure 1:</b> Picture of the desktop spectrometry kit 3.0 from | + | <p class="caption"><b>Figure 1:</b> Picture of the desktop spectrometry kit 3.0 from PublicLab.</p> |
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− | + | 2/ Instructables (Figure 2) gives example: “An Arduino powered microplate spectrophotometer”. This 96 well plate reader is another design inspiration for us.</p> | |
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<p class="caption"><b>Figure 2:</b> An Arduino powered microplate spectrophotometer, from Instructables.</p> | <p class="caption"><b>Figure 2:</b> An Arduino powered microplate spectrophotometer, from Instructables.</p> | ||
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+ | 3/ Another DIY solution came to us in the Journal of Marine Biology. It has a double channel, and it intended to measure pH with an indicator. The data looks great, but we weren’t able to access the source files. Source: Yang et al. (2014). "Seawater pH measurements in the field: A DIY photometer with 0.01 unit pH accuracy". Marine Chemistry. Volume 160, Pages 75–81.</p> | ||
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<img src="https://static.igem.org/mediawiki/2015/8/80/ParisBettencourt_juanma06.jpg" style="width:100%"> | <img src="https://static.igem.org/mediawiki/2015/8/80/ParisBettencourt_juanma06.jpg" style="width:100%"> | ||
<p class="caption"><b>Figure 3:</b> DIY spectrophotometer for pH test from the Marine Chemistry journal.</p> | <p class="caption"><b>Figure 3:</b> DIY spectrophotometer for pH test from the Marine Chemistry journal.</p> | ||
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− | + | 4/ The last example here comes from another fellow iGEM team: iGEM Aachen 2014. Its design is somewhat similar to DI-lambda. They also measured fluorescence. We believe that our design can improve the overall accuracy.</p> | |
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+ | <p> | ||
+ | Overall, while there are very promising projects, it looks like they most of them have been abandoned at early stages, after the initial limited success. The PublicLab spectrometer stands out here, has build instructions, is open-source, and can be purchased as a kit.</p> | ||
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<p class="caption"><b>Figure 4:</b> picture of the iGEM Aachen 2014 spectrophotometer.</p> | <p class="caption"><b>Figure 4:</b> picture of the iGEM Aachen 2014 spectrophotometer.</p> | ||
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− | DI-lambda is an Open Hardware spectrophotometer, designed to be accurate, easy to modify, and extremely cheap. We achieve this by using LEDs instead of an optical refraction system. | + | |
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+ | <h2>Design</h2> | ||
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+ | <div class="column-left"> | ||
+ | <p> | ||
+ | DI-lambda is an Open Hardware spectrophotometer, designed to be accurate, easy to modify, and extremely cheap. We achieve this by using LEDs instead of an optical refraction system.</p> | ||
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+ | <p> | ||
This design choice (Fig. 5) means that the device is limited to one or a few pre-selected wavelengths, but most biological applications only use a few. It is also possible to select a different wavelength by populating a spare LED board, but since it's so cheap, it might be easier to just buy one for each task. | This design choice (Fig. 5) means that the device is limited to one or a few pre-selected wavelengths, but most biological applications only use a few. It is also possible to select a different wavelength by populating a spare LED board, but since it's so cheap, it might be easier to just buy one for each task. | ||
− | Another disadvantage is large spectral width of LEDs, typically 15-25nm, compared to 1-5 nm for traditional spectrophotometers. | + | Another disadvantage is large spectral width of LEDs, typically 15-25nm, compared to 1-5 nm for traditional spectrophotometers.</p> |
− | All this means that DI-lambda is not always able to replace a traditional spectrophotometer, but works great for a majority of biological applications. | + | <p> |
+ | All this means that DI-lambda is not always able to replace a traditional spectrophotometer, but works great for a majority of biological applications.</p> | ||
− | + | The design files are available on Github: https://github.com/lopenlab/OSS-Spectrophotometer/ | |
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+ | <img src="https://static.igem.org/mediawiki/2015/a/ac/ParisBettencourt_juanma08.jpg" style="width: 100%"> | ||
+ | <p class="caption"><b>Figure 5:</b> Diagram of DI-lambda: a) LED b) Sample holder with slits c) Digital light sensor d) Microcontroller e) Computer.</p> | ||
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+ | <h2>Accuracy</h2> | ||
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+ | We put a lot of time into calibrating our spectrophotometer. After tuning parameters, such as the distance between the LED, the sensor, and the cuvette, and the diameter of the pinhole through which the light has to pass, we achieve results that are virtually identical to our most reliable laboratory spectrophotometer. | ||
+ | </p> | ||
+ | </div> | ||
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+ | <img src="https://static.igem.org/mediawiki/2015/4/4c/DI-Lambda_comparision.png" style="width: 100%"> | ||
+ | <p class="caption"><b>Figure 6:</b>Comparision between DI-Lambda and UVline 9100</p> | ||
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+ | <h2>Workshops</h2> | ||
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We keep in mind the importance of interacting with the public and getting feedback from them, at at the same time communicate about science, synthetic biology and citizen science. We collaborated with several institutions and teams to organize events and workshops where participants could test spectrophotometer units… or even build their own units! | We keep in mind the importance of interacting with the public and getting feedback from them, at at the same time communicate about science, synthetic biology and citizen science. We collaborated with several institutions and teams to organize events and workshops where participants could test spectrophotometer units… or even build their own units! | ||
+ | </p> | ||
+ | </div> | ||
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+ | <img src="https://static.igem.org/mediawiki/2015/7/7e/ParisBettencourt_juanma03.jpg" style="width: 100%"> | ||
+ | <p class="caption"><B>Figure 7:</b> DI-lambda Luna. It is a version designed specifically for our workshop at the NightScience 2015 conference held at the Center for Research and Interdisciplinarity in Paris. Largely based on the test rig model, with some added elegance. It is easy to build, and can be used for Do-It-yourself workshops. Search on github to get the complete design! External links are not permitted on wiki.</p> | ||
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+ | NightScience conference at the Center for Research and Interdisciplinarity in Paris was an opportunity in early July to meet 10 testers that built a simplified 1-channel version called DI-lambda Luna (Fig. 6). They had a high interest in electronics and synbio but low knowledge.</p> | ||
+ | <p> | ||
+ | We took some time to cross the English channel and visit our friends at UCL. We spent a few days in London in the iGEM bootcamp organized by the students of UCL with the participation of synbio enthusiastics, high school students from UCL academy, and UCL, Birkbeck and London Hackerspace iGEM teams (Fig. 7). The pictures are just beautiful!</p> | ||
+ | </div> | ||
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+ | <img src="https://static.igem.org/mediawiki/2015/0/08/ParisBettencourt_juanma09.jpg" style="width: 100%"> | ||
+ | <p class="caption"><p>Figure 8:</b> DI-lambda spectrophotometers built in a workshop with UCL, Birkbeck and London Hackerspace iGEM teams organized by UCL.</p> | ||
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− | Please, do not hesitate to contact us if you want further information. | + | |
+ | <h2>What is next?</h2> | ||
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+ | We are planning to make this tool available to more people and be able to mass produce it, keeping a cheap price and simple design. The interesting features of this tool are the low price and the ease to hack it, tune it to a specific need.</p> | ||
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+ | In order to do this tool and many more, this tool will continue integrated in the project Open Science School, at the Center for Research and Interdisciplinarity of Paris.</p> | ||
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+ | <p> | ||
+ | Please, do not hesitate to contact us if you want further information.</p> | ||
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Latest revision as of 03:59, 19 September 2015