Difference between revisions of "Team:Aachen/Lab/Glycogen/Synthesis"
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{{Team:Aachen/Header}} | {{Team:Aachen/Header}} | ||
− | {{Team:Aachen/ReadMore|title=Combined Expression|link=Team:Aachen/Lab/Glycogen/Synthesis#Combined_Expression|picture=rmGlycogenSynthesisIII|url=/ | + | {{Team:Aachen/ReadMore|title=Combined Expression|link=/Team:Aachen/Lab/Glycogen/Synthesis#Combined_Expression|picture=rmGlycogenSynthesisIII|url=/wiki/images/5/50/Aachen_tile_Glycogen_Glycogen_synthesis_combined_expression.JPG}} |
− | {{Team:Aachen/ReadMore|title=Single Expression|link=Team:Aachen/Lab/Glycogen/Synthesis#Single_Expression|picture=rmGlycogenSynthesisII|url=/ | + | {{Team:Aachen/ReadMore|title=Single Expression|link=/Team:Aachen/Lab/Glycogen/Synthesis#Single_Expression|picture=rmGlycogenSynthesisII|url=/wiki/images/0/0c/Aachen_tile_Glycogen_Glycogen_synthesis_single_expression.JPG}} |
− | {{Team:Aachen/ReadMore|title=BioBricks|link=Team:Aachen/Lab/Glycogen/Synthesis#Biobricks|picture=rmGlycogenSynthesisI|url=/ | + | {{Team:Aachen/ReadMore|title=BioBricks|link=/Team:Aachen/Lab/Glycogen/Synthesis#Biobricks|picture=rmGlycogenSynthesisI|url=/wiki/images/b/b2/Aachen_tile_Lab_Glycogen_Glycogen_synthesis_Biobricks.JPG}} |
==How Glycogen Synthesis Works== | ==How Glycogen Synthesis Works== | ||
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The glycogen formation in ''E. coli'' is performed by three enzymes: | The glycogen formation in ''E. coli'' is performed by three enzymes: | ||
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− | {{Team:Aachen/DoubleFigure|Aachen_15-07-09_glgB_expression_test_2.png|Aachen_15-07- | + | {{Team:Aachen/DoubleFigure|Aachen_15-07-09_glgB_expression_test_2.png|Aachen_15-07-17_glgC_expression_test.png|title1=SDS-PAGE of GlgB expression|title2=SDS-PAGE of GlgC expression|subtitle1=GlgB was expressed in pSB1K30 and compared to mRFP expression. The small arrows point to the glgB bands of 84 kDa. No difference between induced and uninduced was observed.|subtitle2=GlgC was expressed in pSB1A30 and compared to mRFP expression. The small arrows point to the glgC bands of 48 kDa. No difference between induced and uninduced was observed. After overnight expression the strong bands were no longer present.|size=medium}} |
<span style="color:transparent">a</span> | <span style="color:transparent">a</span> | ||
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− | {{Team:Aachen/DoubleFigure|Aachen glgCAB and glgAB after IPTG induction 2.png|Aachen glgCAB after IPTG induction | + | {{Team:Aachen/DoubleFigure|Aachen glgCAB and glgAB after IPTG induction 2.png|Aachen glgCAB after IPTG induction 2.png|title1=SDS-PAGE of GlgCAB and GlgAB expression|title2=SDS-PAGE of GlgCAB expression|subtitle1= GlgCAB was expressed in pSB1A30 and GlgAB in pSB1C30. The gel shows gene expression after IPTG induction compared to GlgA in pSB1K30 and BL21 Gold (DE3).|subtitle2=GlgCAB was expressed in pSB1A30 after IPTG induction and compared to the BL21 Gold (DE3). The small arrows indicate the bands of expected protein sizes.|size=medium}} |
<span style="color:transparent">a</span> | <span style="color:transparent">a</span> | ||
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==References== | ==References== | ||
− | <references /> | + | <references/> |
<p id="colorControl" style="display:none;">green</p> | <p id="colorControl" style="display:none;">green</p> | ||
{{Team:Aachen/Footer|color=green}} | {{Team:Aachen/Footer|color=green}} |
Latest revision as of 11:31, 12 October 2015